Fish Collagen Type IV Antibody ELISA Kit | COL-IV Ab elisa kit

Fish Collagen Type IV Antibody ELISA Kit

Cat. Num. AAA12126

• Gene Names: COL4A6; DFNX6; DELXq22.3; CXDELq22.3
• Reactivity: Fish
• Synonyms: Collagen Type IV Antibody; Fish Collagen Type IV Antibody ELISA Kit; COL-IV Ab elisa kit

• Reactivity: Fish
• Specificity: No significant cross-reactivity or interference between Fish COL-IV Ab and analogues was observed.
• Sequence Length: 1633

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 3.12-100ng/ml.
• Sensitivity: 0.5ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated COL-IV Ab concentrations in Fish serum, plasma and other body fluids. Using Purified Fish COL-IV Ab antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add COL-IV Ab and COL-IV Ab antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of COL-IV Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for COL-IV Ab elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated COL-IV Ab concentrations in Fish serum, plasma and other body fluids. Using Purified Fish COL-IV Ab antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add COL-IV Ab and COL-IV Ab antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of COL-IV Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 567757604
• NCBI GeneID: 1288
• NCBI Accession #: NP_001274689.1
• NCBI GenBank Nucleotide #: NM_001287760.1
• UniProt Accession #: Q14031; Q12823; Q14053; Q5JYH6; Q5JYH8; Q9NQM5; Q9NTX3; Q9UJ76; Q9UMG6; Q9Y4L4
• Molecular Weight: 163,807 Da
• NCBI Official Full Name: collagen alpha-6(IV) chain isoform 5
• NCBI Official Synonym Full Names: collagen, type IV, alpha 6
• NCBI Official Symbol: COL4A6
• NCBI Official Synonym Symbols: DFNX6; DELXq22.3; CXDELq22.3
• NCBI Protein Information: collagen alpha-6(IV) chain; collagen alpha-6(IV) chain; collagen IV, alpha-6 polypeptide; dJ889N15.4 (Collagen Alpha 6(IV)); collagen of basement membrane, alpha-6
• UniProt Protein Name: Collagen alpha-6(IV) chain
• UniProt Gene Name: COL4A6
• UniProt Entry Name: CO4A6_HUMAN

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Uniprot Description

COL4A6: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: endoplasmic reticulum lumen; collagen type IV; extracellular region

Molecular Function: extracellular matrix structural constituent

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cell adhesion

Disease: Deafness, X-linked 6