COCH recombinant protein

COCH Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120800

• Gene Names: COCH; DFNA9; COCH5B2; COCH-5B2
• Purity: >92% as determined by SDS-PAGE
• Synonyms: COCH; COCH Protein; Human; Recombinant (His Tag); Human Cochlin/COCH Protein (His Tag); COCH-5B2 Protein; COCH5B2 Protein; DFNA9 Protein; cochlin; COCH recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu25-Gln550

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human COCH (NP_001128530.1)(Glu 25-Gln 550) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for COCH recombinant protein

Background: Cochlin, also known as COCH-5B2 and COCH, is a secreted protein that contains one LCCL domain and two VWFA domains. It is an abundant inner ear protein expressed as multiple isoforms. Its function is also unknown, but it is suspected to be an extracellular matrix component. Cochlin and type II collagen are major constituents of the inner ear extracellular matrix, and Cochlin constitutes 70% of non-collagenous protein in the inner ear, the cochlin isoforms can be classified into three subgroups, p63s, p44s and p40s. The expression of cochlin is highly specific to the inner ear. Eleven missense mutation and one in-frame deletion have been reported in the COCH gene, causing hereditary progressive sensorineural hearing loss and vestibular dysfunction, deafness autosomal dominant type 9 (DFNA9). The co-localization of cochlin and type II collagen in the fibrillar substance in the subepithelial area indicate that cochlin may play a role in the structural homeostasis of the vestibule acting in concert with the fibrillar type II collagen bundles. Defects in COCH may contribute to Meniere disease which is an autosomal dominant disorder characterized by hearing loss associated with episodic vertigo.

References

Ikezono T, et al. (2005) Expression of cochlin in the vestibular organ of rats. ORL J Otorhinolaryngol Relat Spec. 67(5): 252-8.Shindo S, et al. (2008) Spatiotemporal expression of cochlin in the inner ear of rats during postnatal development. Neurosci Lett. 444(2): 148-52.Hosokawa S, et al. (2010) Ultrastructural localization of cochlin in the rat cochlear duct. Audiol Neurootol. 15(4): 247-53.

NCBI and Uniprot Product Information

• NCBI GI #: 205277471
• NCBI GeneID: 1690
• NCBI Accession #: NP_001128530.1
• NCBI GenBank Nucleotide #: NM_001135058.1
• UniProt Accession #: O43405; A8K9K9; D3DS84
• Molecular Weight: 59,483 Da
• NCBI Official Full Name: cochlin
• NCBI Official Synonym Full Names: cochlin
• NCBI Official Symbol: COCH
• NCBI Official Synonym Symbols: DFNA9; COCH5B2; COCH-5B2
• NCBI Protein Information: cochlin; coagulation factor C homolog, cochlin (Limulus polyphemus)
• UniProt Protein Name: Cochlin
• Protein Family: Cochlin
• UniProt Gene Name: COCH
• UniProt Synonym Gene Names: COCH5B2
• UniProt Entry Name: COCH_HUMAN