Mouse anti-Human PODXL Antibody | anti-PODXL antibody
Anti-Hu PODXL Purified
Gene Names
PODXL; PC; PCLP; Gp200; PCLP-1
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Purified by protein-A affinity chromatography.
Synonyms
PODXL; Anti-Hu PODXL Purified; PCLP-1; Gp200; podocalyxin like; anti-PODXL antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG1 kappa
Clone Number
PcMab-47
Specificity
The mouse monoclonal antibody PcMab-47 recognizes a glycosylation-dependent epitope (aa 207-210) on human PODXL, a highly glycosylated transmembrane glycoprotein expressed above all in many types of cancer tissues, and on embryonic stem cells.
Purity/Purification
Purified by protein-A affinity chromatography.
Form/Format
Phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PODXL antibody
Western Blot (WB), Flow Cytometry (FC)(QC tested), Immunohistochemistry (IHC)
Application Notes
FC: Recommended dilution: 1-4ug/ml, extracellular staining
Immunogen
recombinant PODXL ectodomain
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
References
Schulten HJ, Hussein D: Array expression meta-analysis of cancer stem cell genes identifies upregulation of PODXL especially in DCC low expression meningiomas. PLoS One 2019 May 13;14(5):e0215452., URL: https://pubmed.ncbi.nlm.nih.gov/31083655/
Itai S, Yamada S, Kaneko MK, Kato Y: Determination of critical epitope of PcMab-47 against human podocalyxin. Biochem Biophys Rep. 2018 Apr 24;14:78-82., URL: https://pubmed.ncbi.nlm.nih.gov/29872738/
Ogasawara S, Kaneko MK, Yamada S, Honma R, Nakamura T, Saidoh N, Yanaka M, Yoshida K, Fujii Y, Kato Y: PcMab-47: Novel antihuman podocalyxin monoclonal antibody for immunohistochemistry. Monoclon Antib Immunodiagn Immunother. 2017 Apr;36(2):50-56., URL: https://pubmed.ncbi.nlm.nih.gov/28384052/
Itai S, Ohishi T, Kaneko MK, Yamada S, Abe S, Nakamura T, Yanaka M, Chang YW, Ohba SI, Nishioka Y, Kawada M, Harada H, Kato Y: Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma. Oncotarget 2018 Apr 27;9(32):22480-22497., URL: https://pubmed.ncbi.nlm.nih.gov/29854293/
Kaneko MK, Ohishi T, Kawada M, Kato Y: A cancer-specific anti-podocalyxin monoclonal antibody (60-mG 2a-f) exerts antitumor effects in mouse xenograft models of pancreatic carcinoma. Biochem Biophys Rep. 2020 Oct 10;24:100826., URL: https://pubmed.ncbi.nlm.nih.gov/33088928/
Itai S, Yamada S, Kaneko MK, Kato Y: Determination of critical epitope of PcMab-47 against human podocalyxin. Biochem Biophys Rep. 2018 Apr 24;14:78-82., URL: https://pubmed.ncbi.nlm.nih.gov/29872738/
Ogasawara S, Kaneko MK, Yamada S, Honma R, Nakamura T, Saidoh N, Yanaka M, Yoshida K, Fujii Y, Kato Y: PcMab-47: Novel antihuman podocalyxin monoclonal antibody for immunohistochemistry. Monoclon Antib Immunodiagn Immunother. 2017 Apr;36(2):50-56., URL: https://pubmed.ncbi.nlm.nih.gov/28384052/
Itai S, Ohishi T, Kaneko MK, Yamada S, Abe S, Nakamura T, Yanaka M, Chang YW, Ohba SI, Nishioka Y, Kawada M, Harada H, Kato Y: Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma. Oncotarget 2018 Apr 27;9(32):22480-22497., URL: https://pubmed.ncbi.nlm.nih.gov/29854293/
Kaneko MK, Ohishi T, Kawada M, Kato Y: A cancer-specific anti-podocalyxin monoclonal antibody (60-mG 2a-f) exerts antitumor effects in mouse xenograft models of pancreatic carcinoma. Biochem Biophys Rep. 2020 Oct 10;24:100826., URL: https://pubmed.ncbi.nlm.nih.gov/33088928/
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
podocalyxin isoform 1
NCBI Official Synonym Full Names
podocalyxin-like
NCBI Official Symbol
PODXL
NCBI Official Synonym Symbols
PC; PCLP; Gp200; PCLP-1
NCBI Protein Information
podocalyxin; GCTM-2 antigen; podocalyxin-like protein 1
UniProt Protein Name
Podocalyxin
Protein Family
UniProt Gene Name
PODXL
UniProt Synonym Gene Names
PCLP; PCLP1; PC; PCLP-1
UniProt Entry Name
PODXL_HUMAN
Similar Products
Product Notes
The PODXL podxl (Catalog #AAA20036) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu PODXL Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PODXL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC)(QC tested), Immunohistochemistry (IHC). FC: Recommended dilution: 1-4ug/ml, extracellular staining. Researchers should empirically determine the suitability of the PODXL podxl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PODXL, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
