Human Contactin Associated Protein Like Protein 2 ELISA Kit | CNTNAP2 elisa kit

Human Contactin Associated Protein Like Protein 2 ELISA Kit

Cat. Num. AAA760289

• Gene Names: CNTNAP1; P190; CASPR; NRXN4; CNTNAP
• Reactivity: Human
• Synonyms: Contactin Associated Protein Like Protein 2; Human Contactin Associated Protein Like Protein 2 ELISA Kit; CNTNAP2/AUTS15/CASPR2/CDFE/NRXN4/PTHSL1/Cell recognition molecule Caspr2/CDFE/CNTNAP2/NRXN4/AUTS15/CDFE/Cell recognition molecule Caspr2/contactin associated protein-like 2/contactin-associated protein 2/contactin-associated protein-like 2/homolog of Drosophila neurexin IV/NRXN4/Caspr2/PTHSL1; CNTNAP2 elisa kit

• Reactivity: Human
• Sequence Length: 1384

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CNTNAP2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human CNTNAP2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CNTNAP2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CNTNAP2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CNTNAP2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1857708
• NCBI GeneID: 8506
• Molecular Weight: 156,267 Da
• NCBI Official Full Name: contactin associated protein
• NCBI Official Synonym Full Names: contactin associated protein 1
• NCBI Official Symbol: CNTNAP1
• NCBI Official Synonym Symbols: P190; CASPR; NRXN4; CNTNAP
• NCBI Protein Information: contactin-associated protein 1
• UniProt Protein Name: Contactin-associated protein 1
• Protein Family: Contactin-associated protein
• UniProt Gene Name: CNTNAP1
• UniProt Synonym Gene Names: CASPR; NRXN4; Caspr; Caspr1
• UniProt Entry Name: CNTP1_HUMAN

NCBI Description

The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1,384-amino acid protein, also designated p190 or CASPR for 'contactin-associated protein,' includes an extracellular domain with several putative protein-protein interaction domains, a putative transmembrane domain, and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb, with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins, and this protein may be the signaling subunit of contactin, enabling recruitment and activation of intracellular signaling pathways in neurons. [provided by RefSeq, Jan 2009]

Uniprot Description

CNTNAP1: Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells. Belongs to the neurexin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: integral to membrane; integral to plasma membrane; myelin sheath; paranode region of axon; voltage-gated potassium channel complex

Molecular Function: protein binding; receptor activity; SH3 domain binding; SH3/SH2 adaptor activity

Biological Process: action potential propagation; axon guidance; cell adhesion; cytoskeleton organization and biogenesis; neurite morphogenesis; neuromuscular process controlling balance; neuromuscular process controlling posture; paranodal junction assembly; positive regulation of signal transduction; signal transduction

Disease: Lethal Congenital Contracture Syndrome 7