CMV Pp65 recombinant protein
CMV Pp65 protein
Applications
ELISA, Western Blot
Purity
> 95% pure by SDS-PAGE.
Synonyms
CMV Pp65; CMV Pp65 protein; UL83 protein; CMV Pp 65 protein; Cytomegalovirus pp65 Tegument Protein (UL83); CMV pp65 UL83 protein; CMV Pp-65 protein; Cytomegalovirus Pp65 protein; CMV Pp-65; CMV Pp 65; CMV Pp65 recombinant protein
Host
Escherichia Coli
Purity/Purification
> 95% pure by SDS-PAGE.
Form/Format
Liquid in 50mM Tris-HCI with 10mM Glutathione 60mM NaCI, 0.125% Sarcosil and 50% Glycerol.
Concentration
1.0 mg/mL (varies by lot)
Applicable Applications for CMV Pp65 recombinant protein
Suitable for use as an antigen in ELISA and Western Blots, for detection of CMV with minimal specifity problems.
Application Notes
Optimal conditions to be determined by end user.
Protein Type
Recombinant
Biohazard
Use standard laboratory precautions when handling this material.
Biological Significance
CMV belongs to the Beta herpes virinae subfamily of Herpes viridae which includes herpes simplex virus types 1 and 2, varicella-zoster virus, and Epstein-Barr virus. The herpes viruses share a characteristic ability to remain latentover long periods.CMV is a double-stranded linear DNA virus with 162 hexagonal protein capsomeres surrounded by a lipid membrane. CMV has the largest genome of the herpes viruses, ranging from 230-240 kilobase pairs. Human CMV is composed of unique and inverted repeats that include the existence of 4 genome isomers caused by inversion of L-S genome components (class E). Replication may be divided into immediate early, delayed early, and late gene expression based on time of synthesis after infection. The DNA is replicated by rolling circles. In vitro, CMV replicates in human fibroblasts.
Expression System
E Coli
Tag/Conjugate
GST tag
Residues
The E.Coli derived 52.2 kDa recombinant protein contains the CMV Pp65 (UL83) immunodominant regions, 297-510 amino acids. Recombinant CMV-Pp65 is fused to a 26 kDa GST tag
Preparation and Storage
Store at 4°C for short term, -20°C for long term. Avoid freeze-thaws.
Related Product Information for CMV Pp65 recombinant protein
Purified recombinant CMV Pp65 protein
Product Categories/Family for CMV Pp65 recombinant protein
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Product Notes
The CMV Pp65 (Catalog #AAA538067) is a Recombinant Protein produced from Escherichia Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CMV Pp65 can be used in a range of immunoassay formats including, but not limited to, Suitable for use as an antigen in ELISA and Western Blots, for detection of CMV with minimal specifity problems. Optimal conditions to be determined by end user. Researchers should empirically determine the suitability of the CMV Pp65 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CMV Pp65, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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