Collagenase, Type IV, C. histolyticum Enzyme
Collagenase, Type IV, C. histolyticum
Purity
Molecular Biology Grade
Synonyms
Collagenase; Type IV; C. histolyticum; C. histolyticum enzyme
Host
Clostridium histolyticum
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a dialyzed, lyophilized powder.
Application Notes
Suitable for use in pancreatic islet primary isolation and other applications where receptor integrity is crucial. Collagenase is prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity.
Activators
Zinc, Calcium
CAS Number
9001-12-1
Activity
240 units/mg dry weight
Units/mg Caseinase (dw)
210 units/mg dry weight
Units/mg Clostripain (dw)
0.86 units/mg dry weight
Units/mg Trypic (dw)
0.04
Absorbance mg/ml @ 280nm
3.25 mg/ml
Endotoxin EU/mg (dw)
1.92 EU/mg dry weight
Unit Definition
1 unit releases 1umole of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5.
Preparation and Storage
Lyophilized and reconstitutes products are stable for 6 months after receipt at 4°C. Do not freeze. For maximum recovery of product centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Collagenase, Type IV, C. histolyticum enzyme
Collagenase belongs to the peptidase family M10B. Type IV, containing low tryptic activity, is commonly used for islets and other applications where receptor integrity is crucial. It cleaves gelatin Type I and Collagen Types IV, V, VII, X, cleaving the collagen-like: PRO-GLN-GLY-ILE-ALA-GLY-GLN.
Product Categories/Family for Collagenase, Type IV, C. histolyticum enzyme
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Product Notes
The Collagenase, Type IV, C. histolyticum (Catalog #AAA653796) is an Enzyme produced from Clostridium histolyticum and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in pancreatic islet primary isolation and other applications where receptor integrity is crucial. Collagenase is prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. Researchers should empirically determine the suitability of the Collagenase, Type IV, C. histolyticum for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagenase, Type IV, C. histolyticum, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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