CLIC5 recombinant protein

Recombinant Human CLIC5 Protein

Cat. Num. AAA8248802

• Gene Names: CLIC5; MST130; DFNB102; DFNB103; MSTP130
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: CLIC5; Recombinant Human CLIC5 Protein; Chloride intracellular channel protein 5; CLIC5 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: MGSSHHHHHH SSGLVPRGSH MTDSATANGD DRDPEIELFV KAGIDGESIG NCPFSQRLFM ILWLKGVVFN VTTVDLKRKP ADLHNLAPGT HPPFLTFNGD VKTDVNKIEE FLEETLTPEK YPKLAAKHRE SNTAGIDIFS KFSAYIKNTK QQNNAALERG LTKALKKLDD YLNTPLPEEI DANTCGEDKG SRRKFLDGDE LTLADCNLLP KLHVVKIVAK KYRNYDIPAE MTGLWRYLKN AYARDEFTNT CAADSEIELA YADVAKRLSR S
• Sequence Length: 410

• Applicable Applications for CLIC5 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CLIC5 recombinant protein

Recombinant Human CLIC5 Protein is produced by our E.coli expression system and the target gene encoding Met1-Ser251 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 166197662
• NCBI GeneID: 53405
• NCBI Accession #: NP_001107558.1
• NCBI GenBank Nucleotide #: NM_001114086.1
• UniProt Accession #: Q9NZA1; Q5T4Z0; Q8NBY3; Q96JT5; Q9BWZ0; B3KUF1
• Molecular Weight: 22,830 Da
• NCBI Official Full Name: chloride intracellular channel protein 5 isoform a
• NCBI Official Synonym Full Names: chloride intracellular channel 5
• NCBI Official Symbol: CLIC5
• NCBI Official Synonym Symbols: MST130; DFNB102; DFNB103; MSTP130
• NCBI Protein Information: chloride intracellular channel protein 5
• UniProt Protein Name: Chloride intracellular channel protein 5
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: CLIC5
• UniProt Entry Name: CLIC5_HUMAN

NCBI Description

This gene encodes a member of the chloride intracellular channel (CLIC) family of chloride ion channels. The encoded protein associates with actin-based cytoskeletal structures and may play a role in multiple processes including hair cell stereocilia formation, myoblast proliferation and glomerular podocyte and endothelial cell maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CLIC5: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture. Belongs to the chloride channel CLIC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: actin cytoskeleton; nucleus

Molecular Function: chloride channel activity; glutathione transferase activity; protein binding

Biological Process: auditory receptor cell stereocilium organization and biogenesis; chloride transport; female pregnancy; glutathione metabolic process; sensory perception of sound

Disease: Deafness, Autosomal Recessive 103