Fibroblast Growth Factor Binding Protein 1 (FGFBP1) CLIA Kit | FGFBP1 clia kit

CLIA Kit for Fibroblast Growth Factor Binding Protein 1 (FGFBP1)

Cat. Num. AAA2000524

• Gene Names: FGFBP1; FGFBP; HBP17; FGF-BP; FGF-BP1; FGFBP-1
• Synonyms: Fibroblast Growth Factor Binding Protein 1 (FGFBP1); CLIA Kit for Fibroblast Growth Factor Binding Protein 1 (FGFBP1); FGFBP1 clia kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor Binding Protein 1 (FGFBP1); No significant cross-reactivity or interference between Fibroblast Growth Factor Binding Protein 1 (FGFBP1) and analogues was observed.
• Sequence Length: 234

• Assay Type: Sandwich
• Samples: Tissue homogenates and other biological fluids
• Detection Range: 0.027-20ng/mL The standard curve concentrations used for the ELISA's were 20ng/mL, 6.67ng/mL, 2.22ng/mL, 0.74ng/mL, 0.247ng/mL, 0.082ng/mL, 0.027ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.116ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor Binding Protein 1 (FGFBP1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor Binding Protein 1 (FGFBP1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Organism Species: Homo sapiens (Human)
• Assay Length: 4.5 hours
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGFBP1 clia kit

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor Binding Protein 1 (FGFBP1) level in the sample or standard.

NCBI and Uniprot Product Information

• NCBI GI #: 4826754
• NCBI GeneID: 9982
• NCBI Accession #: NP_005121.1
• NCBI GenBank Nucleotide #: NM_005130.4
• UniProt Accession #: Q14512; A8K5J2
• Molecular Weight: 26,264 Da
• NCBI Official Full Name: fibroblast growth factor-binding protein 1
• NCBI Official Synonym Full Names: fibroblast growth factor binding protein 1
• NCBI Official Symbol: FGFBP1
• NCBI Official Synonym Symbols: FGFBP; HBP17; FGF-BP; FGF-BP1; FGFBP-1
• NCBI Protein Information: fibroblast growth factor-binding protein 1; FGF-binding protein 1; 17 kDa HBGF-binding protein; heparin-binding growth factor binding protein; 17 kDa heparin-binding growth factor-binding protein
• UniProt Protein Name: Fibroblast growth factor-binding protein 1
• Protein Family: Fibroblast growth factor-binding protein
• UniProt Gene Name: FGFBP1
• UniProt Synonym Gene Names: FGFBP; HBP17; FGF-BP; FGF-BP1; FGF-binding protein 1; FGFBP-1; 17 kDa HBGF-binding protein; HBp17
• UniProt Entry Name: FGFP1_HUMAN

NCBI Description

This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 4. [provided by RefSeq, Nov 2011]

Uniprot Description

FGFBP1: Acts as a carrier protein that release fibroblast- binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth. Belongs to the fibroblast growth factor-binding protein family.

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: extracellular space; cell surface; plasma membrane

Molecular Function: heparin binding; protein binding; fibroblast growth factor binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; positive regulation of cell proliferation; positive regulation of fibroblast growth factor receptor signaling pathway; signal transduction