Typical Testing Data/Standard Curve (for reference only)
Erythroid Differentiation Related Factor 1 (EDRF1) CLIA Kit | EDRF1 clia kit
CLIA Kit for Erythroid Differentiation Related Factor 1 (EDRF1)
Gene Names
EDRF1; C10orf137
Synonyms
Erythroid Differentiation Related Factor 1 (EDRF1); CLIA Kit for Erythroid Differentiation Related Factor 1 (EDRF1); EDRF1 clia kit
Specificity
This assay has high sensitivity and excellent specificity for detection of Erythroid Differentiation Related Factor 1 (EDRF1)
No significant cross-reactivity or interference between Erythroid Differentiation Related Factor 1 (EDRF1) and analogues was observed.
No significant cross-reactivity or interference between Erythroid Differentiation Related Factor 1 (EDRF1) and analogues was observed.
Sequence Length
1238
Assay Type
Sandwich
Samples
Tissue homogenates and other biological fluids
Detection Range
0.027-20ng/mL The standard curve concentrations used for the ELISA's were 20ng/mL, 6.67ng/mL, 2.22ng/mL, 0.74ng/mL, 0.247ng/mL, 0.082ng/mL, 0.027ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.117ng/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythroid Differentiation Related Factor 1 (EDRF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythroid Differentiation Related Factor 1 (EDRF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythroid Differentiation Related Factor 1 (EDRF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Organism Species
Homo sapiens (Human)
Assay Length
4.5 hours
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EDRF1 clia kit
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Erythroid Differentiation Related Factor 1 (EDRF1) level in the sample or standard.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
138,528 Da
NCBI Official Full Name
erythroid differentiation-related factor 1 isoform 1
NCBI Official Synonym Full Names
erythroid differentiation regulatory factor 1
NCBI Official Symbol
EDRF1
NCBI Official Synonym Symbols
C10orf137
NCBI Protein Information
erythroid differentiation-related factor 1
UniProt Protein Name
Erythroid differentiation-related factor 1
Protein Family
UniProt Gene Name
EDRF1
UniProt Synonym Gene Names
C10orf137
UniProt Entry Name
EDRF1_HUMAN
NCBI Description
This gene may play a role in erythroid cell differentiation. The encoded protein inhibits DNA binding of the erythroid transcription factor GATA-1 and may regulate the expression of alpha-globin and gamma-globin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Uniprot Description
EDRF1: Transcription factor involved in erythroid differentiation. Involved in transcriptional activation of the globin gene. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 10q26.13
Cellular Component: nucleus
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
Research Articles on EDRF1
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Product Notes
The EDRF1 edrf1 (Catalog #AAA2000791) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Erythroid Differentiation Related Factor 1 (EDRF1), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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