MDL-1/CLEC5A recombinant protein

Recombinant MDL-1/CLEC5A

Cat. Num. AAA201085

• Gene Names: CLEC5A; MDL1; MDL-1; CLECSF5
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: MDL-1/CLEC5A; Recombinant MDL-1/CLEC5A; C-type lectin domain family 5 member A isoform 1; CLEC5A; CLECSF5; MDL-1; MDL1; MDL-1/CLEC5A recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 20% Glycerol, 1mM DTT.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 28-188aa
• Sequence: ADLPQIFNKS NDGFTTTRSY GTVSQIFGSS SPSPNGFITT RSYGTVCPKD WEFYQARCFF LSTSESSWNE SRDFCKGKGS TLAIVNTPEK LKFLQDITDA EKYFIGLIYH REEKRWRWIN NSVFNGNVTN QNQNFNCATI GLTKTFDAAS CDISYRRICE KNAKHHHHHH
• Sequence Length: 188

• Applicable Applications for MDL-1/CLEC5A recombinant protein: SDS-PAGE
• Species: Human
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Ala
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for MDL-1/CLEC5A recombinant protein

CLEC5A, also known as C-type lectin domain family 5 member A isoform 1, is a member of the CTL/CTLD superfamily that have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. It functions as a cell attachment receptor for all four serotypes of Dengue virus as well as Japanese encephalitis virus. The binding of dengue virus to CLEC5A triggers signaling through the phosphylation of TYROBP, this interaction does not result in viral entry, but stimulates proinflammatory cytokine release. This protein acts as a positive regulator of osteoclastogenesis and a key regulator of synovial injury and bone erosion during autoimmune joint inflammation. Recombinant human CLEC5A protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for MDL-1/CLEC5A recombinant protein

Immunology

References

Gonzalez-Dominguez E., et al, (2015) J Leukoc Biol. 98:453-466. Chen ST., et al, (2017) Nat Commun. 8:299.

NCBI and Uniprot Product Information

• NCBI GI #: 59797971
• NCBI GeneID: 23601
• UniProt Accession #: Q9NY25
• Molecular Weight: 19.5kDa (170aa); 28-40KDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: C-type lectin domain family 5 member A
• NCBI Official Synonym Full Names: C-type lectin domain containing 5A
• NCBI Official Symbol: CLEC5A
• NCBI Official Synonym Symbols: MDL1; MDL-1; CLECSF5
• NCBI Protein Information: C-type lectin domain family 5 member A
• UniProt Protein Name: C-type lectin domain family 5 member A
• UniProt Gene Name: CLEC5A
• UniProt Synonym Gene Names: CLECSF5; MDL1; MDL-1
• UniProt Entry Name: CLC5A_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein interacts with dnax-activation protein 12 and may play a role in cell activation. Alternative splice variants have been described but their full-length sequence has not been determined. [provided by RefSeq, Jul 2008]