CLEC4E/Mincel Recombinant Protein | CLEC4E recombinant protein

Recombinant Human CLEC4E/Mincel Protein (C-6His)

Cat. Num. AAA2552508

• Gene Names: CLEC4E; MINCLE; CLECSF9
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: CLEC4E/Mincel; Recombinant Human CLEC4E/Mincel Protein (C-6His); C-Type Lectin Domain Family 4 Member E; C-Type Lectin Superfamily Member 9; Macrophage-Inducible C-Type Lectin; CLEC4E; CLECSF9; MINCLE; CLEC4E recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence Positions: Arg41-Leu219
• Sequence Length: 219

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-6His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for CLEC4E recombinant protein

C-Type Lectin Domain Family 4 Member E (CLEC4E) is a 219 amino acid single-pass type II membrane protein that contains one C-type Lectin domain. It is expressed in monocytes, CLEC4E functions as a downstream target of C/EBP beta and is thought to play a role in the inflammatory response, possibly via transcriptional control of C/EBP beta. CLEC4E may play a role in the response to inflammatory stimuli in peritoneal macrophages and may be involved in immune surveillance processes under transcriptional control of CEBPB. Human CLEC4E shares 67% sequence identity with its mouse counterpart, suggesting a similar function between species. CLEC-4E exists as multiple alternatively spliced isoforms that are encoded by a gene which maps to a natural killer gene complex region on human chromosome 12.

NCBI and Uniprot Product Information

• NCBI GI #: 7657333
• NCBI GeneID: 26253
• NCBI Accession #: NP_055173.1
• NCBI GenBank Nucleotide #: NM_014358.3
• UniProt Accession #: Q9ULY5; B2R6Q6
• Molecular Weight: Molecular Mass: 21.7 kDa; Actual Protein Molecular Mass: 26 kDa
• NCBI Official Full Name: C-type lectin domain family 4 member E
• NCBI Official Synonym Full Names: C-type lectin domain family 4 member E
• NCBI Official Symbol: CLEC4E
• NCBI Official Synonym Symbols: MINCLE; CLECSF9
• NCBI Protein Information: C-type lectin domain family 4 member E
• UniProt Protein Name: C-type lectin domain family 4 member E
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC4E
• UniProt Synonym Gene Names: MINCLE1 PublicationManual assertion based on opinion iniRef.10

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

Uniprot Description

A calcium-dependent lectin that acts as a pattern recognition receptor of the innate immune system. Recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi (PubMed:18509109, PubMed:23602766). The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions (PubMed:23602766, PubMed:24101491). Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation. Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue ().