Human Claudin 9 ELISA Kit | CLDN9 elisa kit

Human Claudin 9 ELISA Kit

Cat. Num. AAA764479

• Reactivity: Human
• Synonyms: Claudin 9; Human Claudin 9 ELISA Kit; CLDN9/claudin 9/claudin-9; CLDN9 elisa kit

• Reactivity: Human
• Sequence Length: 217

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CLDN9 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human CLDN9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CLDN9 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CLDN9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman CLDN9 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 11141861
• NCBI GeneID: 9080
• NCBI Accession #: NP_066192.1
• NCBI GenBank Nucleotide #: NM_020982.3
• Molecular Weight: 22,848 Da
• NCBI Official Full Name: claudin-9
• NCBI Official Synonym Full Names: claudin 9
• NCBI Official Symbol: CLDN9
• NCBI Protein Information: claudin-9
• UniProt Protein Name: Claudin-9
• Protein Family: Claudin
• UniProt Gene Name: CLDN9
• UniProt Entry Name: CLD9_HUMAN

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This protein is one of the entry cofactors for hepatitis C virus. Mouse studies revealed that this gene is required for the preservation of sensory cells in the hearing organ and the gene deficiency is associated with deafness. [provided by RefSeq, Jun 2010]

Uniprot Description

Claudin-9: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: integral to membrane; plasma membrane; tight junction

Molecular Function: identical protein binding; structural molecule activity

Biological Process: calcium-independent cell-cell adhesion; intercellular junction assembly and maintenance; viral reproduction