Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using Claudin 5 (Phospho-Tyr217) antibody.)
Claudin 5 Antibody | anti-CLDN5 antibody
Claudin 5 (Phospho-Tyr217) Antibody
Gene Names
CLDN5; AWAL; BEC1; TMVCF; TMDVCF; CPETRL1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
Claudin 5; Claudin 5 (Phospho-Tyr217) Antibody; CLD5; TMDVCF; Transmembrane protein deleted in VCFS; anti-CLDN5 antibody
Reactivity
Human, Mouse, Rat
Specificity
Claudin 5 (Phospho-Tyr217) antibody detects endogenous levels of Claudin 5 only when phosphorylated at tyrosine 217.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
303
Applicable Applications for anti-CLDN5 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1000
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Claudin 5 around the phosphorylation site of tyrosine 217 (K-N-YP-V).
All Sites
Human: Tyr217
Mouse: Tyr217
Rat: Tyr217
Mouse: Tyr217
Rat: Tyr217
Preparation and Storage
Store at-20 degree C for 1 year.
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using Claudin 5 (Phospho-Tyr217) antibody.)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using Claudin 5 (Phospho-Tyr217) antibody.)
Product Categories/Family for anti-CLDN5 antibody
References
Sirotkin H., Genomics 42:245-251(1997). Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004). The MGC Project Team; Genome Res. 14:2121-2127(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,147 Da
NCBI Official Full Name
claudin-5 isoform 1
NCBI Official Synonym Full Names
claudin 5
NCBI Official Symbol
CLDN5
NCBI Official Synonym Symbols
AWAL; BEC1; TMVCF; TMDVCF; CPETRL1
NCBI Protein Information
claudin-5
UniProt Protein Name
Claudin-5
Protein Family
UniProt Gene Name
CLDN5
UniProt Synonym Gene Names
AWAL; TMVCF; TMDVCF
NCBI Description
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]
Uniprot Description
Plays a major role in tight junction-specific obliteration of the intercellular space.
Research Articles on CLDN5
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Product Notes
The CLDN5 cldn5 (Catalog #AAA852368) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Claudin 5 (Phospho-Tyr217) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin 5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50-1:100 ELISA: 1:1000. Researchers should empirically determine the suitability of the CLDN5 cldn5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin 5, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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