Typical Testing Data/Standard Curve (for reference only)
Sheep Chloride Channel Protein 1 ELISA Kit | CLC1 elisa kit
Sheep Chloride Channel Protein 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CKS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CKS2 antibody and an CKS2-HRP conjugate. The assay sample and buffer are incubated together with CKS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CKS2 concentration since CKS2 from samples and CKS2-HRP conjugate compete for the anti-CKS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CKS2 from the sample, fewer sites are left to bind CKS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CKS2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. The protein encoded by this gene regulates the electric excitability of the skeletal muscle membrane. Mutations in this gene cause two forms of inherited human muscle disorders: recessive generalized myotonia congenita (Becker) and dominant myotonia (Thomsen). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]
Uniprot Description
CLCN1: Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. Defects in CLCN1 are the cause of Thomsen disease (THD); also known as autosomal dominant myotonia congenita (MCD). THD is characterized by skeletal muscle stiffness (delayed relaxation), due to membrane hyperexcitability. A variant form of Thomsen disease is myotonia levior that is characterized by milder symptoms, later onset and absence of muscle hypo- and hypertrophy. Defects in CLCN1 are the cause of autosomal recessive myotonia congenita (MCR); also known as Becker disease. Belongs to the chloride channel (TC 2.A.49) family. ClC-1/CLCN1 subfamily.
Protein type: Membrane protein, integral; Transporter, ion channel; Membrane protein, multi-pass; Transporter; Channel, chloride
Chromosomal Location of Human Ortholog: 7q35
Cellular Component: integral to plasma membrane; plasma membrane; sarcolemma
Molecular Function: chloride channel activity; voltage-gated chloride channel activity
Biological Process: muscle contraction; transport; action potential propagation; transmembrane transport
Disease: Myotonia Congenita, Autosomal Recessive; Myotonia Congenita, Autosomal Dominant
Research Articles on CLC1
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Product Notes
The Sheep CLC1 clcn1 (Catalog #AAA744913) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA744913 ELISA Kit recognizes Sheep CLC1. It is sometimes possible for the material contained within the vial of "Chloride Channel Protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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