Creatine Kinase BB/CKB Recombinant Protein | CKB recombinant protein

Recombinant Human Creatine Kinase BB/CKB Protein (N-His)

Cat. Num. AAA2557317

• Gene Names: CKB; BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
• Purity: >90% as determined by reducing SDS-PAGE.
• Synonyms: Creatine Kinase BB/CKB; Recombinant Human Creatine Kinase BB/CKB Protein (N-His); B-CK; CKB; Creatine Kinase BB; CKB recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 20mM TrisHCl, 150mM NaCl, 10% Glycerol, pH 7.5.
• Sequence: Met1-Lys381
• Sequence Length: 381

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: N-His
• Preparation and Storage: Store at < -20 degree C, stable for 6 months. Please minimize freeze-thaw cycles.

Related Product Information for CKB recombinant protein

Protein Construction: Recombinant Human Creatine Kinase BB is produced by our E Coli expression system and the target gene encoding Met1-Lys381 is expressed with a 6His tag at the N-terminus.

Background: Creatine kinase B-type (CKB) belongs to the ATP:guanido phosphotransferase family. It has dimer of identical or non-identical chains with MM being the major form in skeletal muscle and myocardium. MB exists in myocardium, and BB exists in many tissues, especially brain. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure.

NCBI and Uniprot Product Information

• NCBI GI #: 125294
• NCBI GeneID: 1152
• UniProt Accession #: P12277
• Molecular Weight: Molecular Mass: 44.8kDa; Actual Protein Molecular Mass: 50kDa
• NCBI Official Full Name: Creatine kinase B-type
• NCBI Official Synonym Full Names: creatine kinase B
• NCBI Official Symbol: CKB
• NCBI Official Synonym Symbols: BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
• NCBI Protein Information: creatine kinase B-type
• UniProt Protein Name: Creatine kinase B-type
• Protein Family: Creatine kinase
• UniProt Gene Name: CKB
• UniProt Synonym Gene Names: CKBB
• UniProt Entry Name: KCRB_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

CKB: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: mitochondrion; cytosol

Molecular Function: protein binding; creatine kinase activity; ATP binding

Biological Process: substantia nigra development; phosphorylation; creatine metabolic process; cellular chloride ion homeostasis