Typical Testing Data/Standard Curve (for reference only)
Porcine CytoKeratin 18 ELISA Kit | CK 18 elisa kit
Porcine CytoKeratin 18 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CY18 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CY18 antibody and an CY18-HRP conjugate. The assay sample and buffer are incubated together with CY18-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CY18 concentration since CY18 from samples and CY18-HRP conjugate compete for the anti-CY18 antibody binding site. Since the number of sites is limited, as more sites are occupied by CY18 from the sample, fewer sites are left to bind CY18-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CY18 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.
Protein type: Cytoskeletal; Nucleolus
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: perinuclear region of cytoplasm; cytoplasm; keratin filament; nucleolus; intermediate filament; microtubule organizing center
Molecular Function: protein binding; structural molecule activity
Biological Process: anatomical structure morphogenesis; tumor necrosis factor-mediated signaling pathway; viral reproduction; intermediate filament cytoskeleton organization and biogenesis; cell cycle; negative regulation of apoptosis; Golgi to plasma membrane CFTR protein transport
Disease: Cirrhosis, Familial
Research Articles on CK 18
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Product Notes
The Porcine CK 18 krt18 (Catalog #AAA741736) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741736 ELISA Kit recognizes Porcine CK 18. It is sometimes possible for the material contained within the vial of "CytoKeratin 18, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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