CIRBP recombinant protein

CIRBP Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121739

• Gene Names: CIRBP; CIRP
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CIRBP; CIRBP Protein; Human; Recombinant (His Tag); Human CIRBP/Cold-inducible RNA-binding Protein (His Tag); CIRP Protein; cold inducible RNA binding protein; CIRBP recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 0.25M Imi, 20% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu172

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human CIRBP (Q14011)(Met1-Glu172) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CIRBP recombinant protein

Background: CIRBP, also known as cold-inducible RNA-binding protein, plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. CIRBP responds to a wide array of cellular stresses, including short wavelength ultraviolet light (UVC), at the transcriptional and post-translational level. It acts as a translational activator.CIRBP can bind the 3 translated region of specific transcripts to stabilize them and facilitate their transport to ribosomes for translation. CIRBP affects NF-kappaB signaling as opposed to IL1B mRNA stability directly.

References

Artero-Castro A. et al., 2009, Mol Cell Biol. 29 (7): 1855-68.Zeng Y. et al., 2009, J Cell Biochem. 107 (1): 179-88.Brochu C. et al., 2013, PLoS One. 8 (2): e57426.

NCBI and Uniprot Product Information

• NCBI GI #: 5921786
• NCBI GeneID: 1153
• UniProt Accession #: Q14011; B3KT17; B4E2X2
• Molecular Weight: 172
• NCBI Official Full Name: Cold-inducible RNA-binding protein
• NCBI Official Synonym Full Names: cold inducible RNA binding protein
• NCBI Official Symbol: CIRBP
• NCBI Official Synonym Symbols: CIRP
• NCBI Protein Information: cold-inducible RNA-binding protein; A18 hnRNP; glycine-rich RNA binding protein; cold inducible RNA-binding protein
• UniProt Protein Name: Cold-inducible RNA-binding protein
• Protein Family: Cold-inducible RNA-binding protein
• UniProt Gene Name: CIRBP
• UniProt Synonym Gene Names: A18HNRNP; CIRP
• UniProt Entry Name: CIRBP_HUMAN

Uniprot Description

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (). Promotes assembly of stress granules (SGs), when overexpressed.