CHRNA2 / Neuronal Acetylcholine Receptor Subunit Alpha-2 Recombinant Protein | CHRNA2 recombinant protein

Human CHRNA2 / Neuronal Acetylcholine Receptor Subunit Alpha-2 Recombinant Protein

• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: CHRNA2 / Neuronal Acetylcholine Receptor Subunit Alpha-2; Human CHRNA2 / Neuronal Acetylcholine Receptor Subunit Alpha-2 Recombinant Protein; Neuronal acetylcholine receptor subunit alpha-2; CHRNA2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 529

• Applicable Applications for CHRNA2 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Neuronal AChR seems to be composed of two different types of subunits: alpha and non-alpha (beta). Alpha-2 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors. The alpha-2:beta-2 nAChR complex is proposed to be a heteropentamer with two subtypes: LS (low agonist sensitivity) with a (alpha-2)3:(beta-2)2 and HS (high agonist sensitivity) with a (alpha-2)2:(beta-2)3 stoichiometry; the subtypes differ in their subunit binding interfaces which are involved in ligand binding.
• Entry Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 153792669
• NCBI GeneID: 1135
• NCBI Accession #: NP_000733
• NCBI GenBank Nucleotide #: NP_000733.2
• UniProt Accession #: Q15822
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-2 isoform 1
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic alpha 2 subunit
• NCBI Official Symbol: CHRNA2
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-2
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-2
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNA2
• UniProt Entry Name: ACHA2_HUMAN

NCBI Description

Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq, Nov 2009]