Standard Curve (Sample)
Mouse C4 Binding Protein, Alpha ELISA Kit | C4BPa elisa kit
Mouse C4 Binding Protein, Alpha ELISA Kit
Gene Names
C4BPA; PRP; C4BP
Reactivity
Mouse
Synonyms
C4 Binding Protein, Alpha; N/A; Mouse C4 Binding Protein, Alpha ELISA Kit; C4BPa elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
5.0-100ng/mL
Sensitivity
1.0ng/mL
Intended Uses
This C4BPa ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C4BPa. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for C4BPa elisa kit
Principle of the Assay: C4BPa ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C4BPa antibody and an C4BPa-HRP conjugate. The assay sample and buffer are incubated together with C4BPa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C4BPa concentration since C4BPa from samples and C4BPa-HRP conjugate compete for the anti-C4BPa antibody binding site. Since the number of sites is limited, as more sites are occupied by C4BPa from the sample, fewer sites are left to bind C4BPa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4BPa concentration in each sample is interpolated from this standard curve.
Product Categories/Family for C4BPa elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,033 Da
NCBI Official Full Name
C4b-binding protein alpha chain
NCBI Official Synonym Full Names
complement component 4 binding protein, alpha
NCBI Official Symbol
C4BPA
NCBI Official Synonym Symbols
PRP; C4BP
NCBI Protein Information
C4b-binding protein alpha chain; proline-rich protein
UniProt Protein Name
C4b-binding protein alpha chain
UniProt Gene Name
C4BPA
UniProt Synonym Gene Names
C4BP; C4bp; PRP
UniProt Entry Name
C4BPA_HUMAN
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Product Notes
The Mouse C4BPa c4bpa (Catalog #AAA89494) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA89494 ELISA Kit recognizes Mouse C4BPa. It is sometimes possible for the material contained within the vial of "C4 Binding Protein, Alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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