CRACC Active Protein
CRACC, Recombinant, Mouse (CD2-like Receptor-Activating Cytotoxic Cells, SLAMF7)
Purity
Highly Purified95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
CRACC; Recombinant; Mouse (CD2-like Receptor-Activating Cytotoxic Cells; SLAMF7); CRACC active protein
Host
CHO Recombinant Mouse
Purity/Purification
Highly Purified
95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.
Activity
Measured by its ability to bind biotinylated rmCRACC.
Endotoxin Level
1EU/1ug
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for CRACC active protein
CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 66kD type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature mouse CRACC consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). Within the ECD, mouse CRACC shares 53% aa sequence identity with human CRACC. It shares 19%-35% aa sequence identity with comparable regions of other mouse SLAM proteins including 2B4, BLAME, CD2F-10, CD84, CD229, NTB-A, and SLAM/CD150. Additional isoforms of mouse CRACC are distinguished by deletions and/or substitutions in their cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8+ T cells, activated B cells, and mature dendritic cells (4, 5). It interacts homophilically to induce NK, CTL, and B cell activation (4-7). In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2 (8).
A DNA sequence encoding the extracellular domain of mouse CRACC (Ala 22-Gly 224; Accession # Q8BHK6) was fused to a 10X histidine tag at the C-terminus. The protein was expressed in Chinese Hamster Ovary cells.
Calculated molecular mass of 23.6kD. As a result of glycosylation, the recombinant protein migrates as an ~ 34-55kD protein in SDS-PAGE under reducing conditions.
A DNA sequence encoding the extracellular domain of mouse CRACC (Ala 22-Gly 224; Accession # Q8BHK6) was fused to a 10X histidine tag at the C-terminus. The protein was expressed in Chinese Hamster Ovary cells.
Calculated molecular mass of 23.6kD. As a result of glycosylation, the recombinant protein migrates as an ~ 34-55kD protein in SDS-PAGE under reducing conditions.
Product Categories/Family for CRACC active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
CD2-like receptor activating cytotoxic cells
Uniprot Description
SLAMF7: Isoform 1 mediates NK cell activation through a SH2D1A- independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q23.1-q24.1
Cellular Component: integral to membrane
Molecular Function: receptor activity
Biological Process: natural killer cell mediated cytotoxicity; natural killer cell activation; cell adhesion
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Product Notes
The CRACC (Catalog #AAA636722) is an Active Protein produced from CHO Recombinant Mouse and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CRACC, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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