FAS Ligand, soluble Active Protein | FAS Ligand active protein

Human FAS Ligand, soluble

Cat. Num. AAA691904

• Gene Names: FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
• Reactivity: Human
• Purity: >= 95% by SDS-PAGE gel and HPLC analyses
• Synonyms: FAS Ligand; soluble; Human FAS Ligand; Recombinant Human sFas Ligand; FAS Ligand active protein

• Host: CHO Cells
• Reactivity: Human
• Purity/Purification: >= 95% by SDS-PAGE gel and HPLC analyses
• Form/Format: Lyophilized
• Sequence: HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLL SGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN S KYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVS ELS LVNFEESQTF FGLYKL
• Sequence Length: 281

• Endotoxin Level: < 0.1 ng per ug of sFas Ligand
• Label/Conjugation: His-Tag
• Biological Activity: Determined by it\'s ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is <= 10.0 ng/ml, corresponding to a specific activity of >= 1 x 105 units/mg.

Related Product Information for FAS Ligand active protein

Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant human soluble Fas Ligand is a 17.9 kDa protein (175 amino acid residues) comprising the TNF homologous region of FasL plus an eight His-Tag.

Product Categories/Family for FAS Ligand active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4557329
• NCBI GeneID: 356
• NCBI Accession #: NP_000630.1
• NCBI GenBank Nucleotide #: NM_000639.1
• UniProt Accession #: P48023; Q9BZP9
• Molecular Weight: 17.9 kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 6
• NCBI Official Synonym Full Names: Fas ligand (TNF superfamily, member 6)
• NCBI Official Symbol: FASLG
• NCBI Official Synonym Symbols: APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 6; CD95 ligand; fas antigen ligand; apoptosis antigen ligand; apoptosis (APO-1) antigen ligand 1; tumor necrosis factor (ligand) superfamily, member 6
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 6
• UniProt Gene Name: FASLG
• UniProt Synonym Gene Names: APT1LG1; CD95L; FASL; TNFSF6; APTL; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA
• UniProt Entry Name: TNFL6_HUMAN

NCBI Description

The protein encoded by this gene is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with this ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Ref.12The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition. Ref.12

Subunit structure: Homotrimer

Probable. Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Ref.13 Ref.14

Subcellular location: Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen. Lysosome lumen. Note: Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane. Ref.12 Ref.13Tumor necrosis factor ligand superfamily member 6, soluble form: Secreted

By similarity. Note: May be released into the extracellular fluid, probably by cleavage form the cell surface

By similarity. Ref.12 Ref.13FasL intracellular domain: Nucleus. Note: The FasL ICD cytoplasmic form is translocated into the nucleus. Ref.12 Ref.13

Post-translational modification: The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.N-glycosylated.Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization. Ref.13Monoubiquitinated.

Involvement in disease: Autoimmune lymphoproliferative syndrome 1B (ALPS1B) [MIM:601859]: A disorder of apoptosis that manifests in early childhood and results in the accumulation of autoreactive lymphocytes. It is characterized by non-malignant lymphadenopathy with hepatosplenomegaly, and autoimmune hemolytic anemia, thrombocytopenia and neutropenia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9

Sequence similarities: Belongs to the tumor necrosis factor family.