Thrombopoietin Active Protein | THPO active protein

Thrombopoietin, Recombinant, Human (TPO, Mpl-ligand, MGDF)

Cat. Num. AAA650901

• Gene Names: THPO; ML; TPO; MGDF; MKCSF; MPLLG; MGC163194
• Purity: Highly Purified; ~ 97% as determined by Analysis by RP-HPLC, Anion-exchange FPLC or Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
• Synonyms: Thrombopoietin; Recombinant; Human (TPO; Mpl-ligand; MGDF); THPO active protein

• Host: CHO cells
• Purity/Purification: Highly Purified; ~ 97% as determined by Analysis by RP-HPLC, Anion-exchange FPLC or Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
• Form/Format: Supplied as a lyophilized powder in 20mM sodium citrate buffer, pH 6.9. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to 100ug/ml, which can then be further diluted to other aqueous solutions.
• Sequence: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSG LLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELLNGTRGLF PGPSRRTLGA PDISSGTSDT GSLPPNLQPG YSPSPTHPPT GQYTLFPLPP TLPTPVVQLH PLLPDPSAPT PTPTSPLLNT SYTHSQNLSQ EG

• Biological Activity: rHuTPO is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of MO7e cells is 1-3 ng/ml, corresponding to a Specific Activity of 1 x 106 IU/mg.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months. Reconstitute with ddH2O and carrier protein (0.1% HSA or BSA). Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for THPO active protein

Thrombopoietin (Tpo), the ligand for the receptor encoded by the c-Mpl proto-oncogene, is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. The cDNAs for Tpo have recently been cloned from canine, murine and human sources. The proteins from these three species are highly conserved, exhibiting from 69-75% sequence identity at the amino acid level. Human Tpo cDNA encodes a 353 amino acid residue protein with a 21 amino acid residue signal peptide that is cleaved to yield the 332 amino acid residue mature protein. Two distinct domains, separated by a pair of arginine residues that may be a proteolytic cleavage site, have been identified in Tpo: the amino terminal region exhibiting sequence homology to erythropoietin and the carboxy terminal region containing multiple potential N-linked glycosylation sites. Recombinant Tpo has now been shown to stimulate the maturation, as well as the proliferation, of megakaryocytes and may have important therapeutic applications for the treatment of various clinical conditions associated with thrombocytopenia. Recombinant Human TPO is approximately 80kD, consisting of a 332 amino acid residue with a predicted molecular mass of approximately 35kD. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 80±10kD in SDS-PAGE.

Product Categories/Family for THPO active protein

Molecular Biology; MB-Thrombopoietin

NCBI and Uniprot Product Information

• NCBI GI #: 914226
• NCBI GeneID: 7066
• UniProt Accession #: P40225; Q13020; Q15790; Q15791; Q15792; A1L3Y0; B7ZLR8; B9EGA8; O94784; Q5FBX4; Q5FBX6; Q5FBX8
• Molecular Weight: ~80kD
• NCBI Official Full Name: thrombopoietin
• NCBI Official Synonym Full Names: thrombopoietin
• NCBI Official Symbol: THPO
• NCBI Official Synonym Symbols: ML; TPO; MGDF; MKCSF; MPLLG; MGC163194
• NCBI Protein Information: thrombopoietin; MPL ligand; c-mpl ligand; OTTHUMP00000210502; OTTHUMP00000210503; OTTHUMP00000210504; thrombopoietin nirs variant 1; megakaryocyte stimulating factor; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand
• UniProt Protein Name: Thrombopoietin
• Protein Family: Thrombopoietin
• UniProt Gene Name: THPO
• UniProt Synonym Gene Names: MGDF
• UniProt Entry Name: TPO_HUMAN

NCBI Description

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Alternate splicing results in multiple transcript variants of this gene.

Uniprot Description

Function: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

Subcellular location: Secreted.

Domain: Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.

Involvement in disease: Defects in THPO are a cause of essential thrombocythemia (ET) [

MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Ref.10

Sequence similarities: Belongs to the EPO/TPO family.