WNT9B active protein

WNT9B

Cat. Num. AAA553628

• Gene Names: WNT9B; WNT15; WNT14B
• Applications: SDS-Page
• Purity: >95%, as determined by SDS-PAGE and HPLC.
• Synonyms: WNT9B; WNT9B active protein

• Host: CHO
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC.
• Form/Format: Lyophilized; PBS, pH 7.2
• Sequence: SYFGLTGREV LTPFPGLGTA AAPAQGGAHL KQCDLLKLSR RQKQLCRREP GLAETLRDAA HLGLLECQFQ FRHERWNCSL EGRMGLLKRG FKETAFLYAV SSAALTHTLA RACSAGRMER CTCDDSPGLE SRQAWQWGVC GDNLKYSTKF LSNFLGSKRG NKDLRARADA HNTHVGIKAV KSGLRTTCKC HGVSGSCAVR TCWKQLSPFR ETGQVLKLRY DSAVKVSSAT NEALGRLELW APARQGSLTK GLAPRSGDLV YMEDSPSFCR PSKYSPGTAG RVCSREASCS SLCCGRGYDT QSRLVAFSCH CQVQWCCYVE CQQCVQEELV YTCKH
• Sequence Length: 357

• Applicable Applications for WNT9B active protein: SDS-PAGE
• Application Notes: Recombinant WNT9B migrates as an approximately 37 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
• Species: Human
• Source Note: Optimized DNA sequence encoding Human WNT9B mature chain including a C-terminal 6xHis tag was expressed in CHO. Recombinant Human Protein Wnt-9b encodes amino acid residues 23-357.
• Biological Activity: Determined by its ability to induce alkaline phosphatase production by CCL-226 cells.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 37181546
• NCBI GeneID: 7484
• Molecular Weight: 39,001 Da
• NCBI Official Full Name: WNT9B
• NCBI Official Synonym Full Names: Wnt family member 9B
• NCBI Official Symbol: WNT9B
• NCBI Official Synonym Symbols: WNT15; WNT14B
• NCBI Protein Information: protein Wnt-9b
• UniProt Protein Name: Protein Wnt-9b
• Protein Family: Protein
• UniProt Gene Name: WNT9B

NCBI Description

The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal embryonic kidney development, and for normal development of the urogenital tract, including uterus and part of the oviduct and the upper vagina in females, and epididymis and vas deferens in males. Activates a signaling cascade in the metanephric mesenchyme that induces tubulogenesis. Acts upstream of WNT4 in the signaling pathways that mediate development of kidney tubules and the Muellerian ducts. Plays a role in cranofacial development and is required for normal fusion of the palate during embryonic development ().