EphA4 Receptor Activation Antibody Sampler Kit | EphA4 antibody sampler kit
EphA4 Receptor Activation
Reactivity
Human, Rat, Mouse, Chicken
Applications
Western Blot, ELISA
Synonyms
EphA4 Receptor Activation; EphA4 antibody sampler kit
Reactivity
Human, Rat, Mouse, Chicken
Form/Format
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for EphA4 antibody sampler kit
Western Blot (WB), ELISA (EIA)
Preparation and Storage
Store all at -20 degree C.Stable for 1 year.
Related Product Information for EphA4 antibody sampler kit
Kit Summary: The EphA4 activation antibody sampler kit can be used to detect EphA4 phosphorylation of Tyr-602 and Tyr-779. The kitalso includes rabbit polyclonal and mouse monoclonal antibodies to examine total EphA4 expression levels andsecondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.
Background: The EpHfamily of Receptor tyrosine kinases and their Ephrin ligands are important for cell positioning andmorphogenesis during development. EpHreceptors are classified into 10 EphA and 6 EphB receptors, whichpreferentially bind to the type A and type B ephrins, respectively. The EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. EphA4 signaling pathways require its kinase activity andinvolve binding and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). EphA4 activation leadsautophosphorylation of Tyr-596 and Tyr-602, and the conserved sites in EphA2 are required for binding to the GEFs, Vav2 and Vav3, and ephrin-induced cell migration. The Tyr-779 site in the kinase domain is also phosphorylated in vivoand may regulate kinase activity. Activated EphA4 leads to Src kinase phosphorylation of the GEF, ephexin-1, and this activates RhoA. Thus, EphA4 signaling involves complex tyrosine phosphorylation in its cytoplasmic region along with interaction with several GEFs.
Background: The EpHfamily of Receptor tyrosine kinases and their Ephrin ligands are important for cell positioning andmorphogenesis during development. EpHreceptors are classified into 10 EphA and 6 EphB receptors, whichpreferentially bind to the type A and type B ephrins, respectively. The EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. EphA4 signaling pathways require its kinase activity andinvolve binding and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). EphA4 activation leadsautophosphorylation of Tyr-596 and Tyr-602, and the conserved sites in EphA2 are required for binding to the GEFs, Vav2 and Vav3, and ephrin-induced cell migration. The Tyr-779 site in the kinase domain is also phosphorylated in vivoand may regulate kinase activity. Activated EphA4 leads to Src kinase phosphorylation of the GEF, ephexin-1, and this activates RhoA. Thus, EphA4 signaling involves complex tyrosine phosphorylation in its cytoplasmic region along with interaction with several GEFs.
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Product Notes
The Human, Rat, Mouse, Chicken EphA4 (Catalog #AAA474309) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474309 Antibody Sampler Kit recognizes Human, Rat, Mouse, Chicken EphA4. AAA Biotech's EphA4 Receptor Activation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the EphA4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EphA4 Receptor Activation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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