CXCR4 Phospho-Regulation Antibody Sampler Kit | CXCR4 antibody sampler kit
CXCR4 Phospho-Regulation
Reactivity
Human, Rat, Mouse, Chicken
Applications
Western Blot, ELISA
Synonyms
CXCR4 Phospho-Regulation; CXCR4 antibody sampler kit
Reactivity
Human, Rat, Mouse, Chicken
Form/Format
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for CXCR4 antibody sampler kit
Western Blot (WB), ELISA (EIA)
Preparation and Storage
Store all at -20 degree C. Stable for 1year.
Related Product Information for CXCR4 antibody sampler kit
Kit Summary: The CXCR4 phospho-regulation antibody sampler kit can be used to examine the level of dual phosphorylation of Ser-324/Ser-325 relative to the total CXCR4 expression level in western blot, ELISA, and immunocytochemistry.
Background: The chemokine receptor CXCR4 is a widely expressed G protein-coupled receptor required for development, hematopoiesis, organogenesis, and vascularization. In disease, CXCR4 has been implicated in WHIM syndrome, HIV, and cancers. Regulation of CXCR4 function occurs through phosphorylation at multiple sites in the C-terminal region. These sites have been shown to be phosphorylated after CXCL12 stimulation, and involve several kinases, such as PKCand GRK kinases. After CXCL12 stimulation of HEK293 cells, Ser-324 and Ser-325 become phosphorylated by PKC andGRK6, while Ser-330 and Ser-339 are phosphorylated by only GRK6. In human astroglia cells, Ser-324 and Ser-325 are rapidly phosphorylated in endogenous CXCR4, while Ser-330 was phosphorylated with slower kinetics. In addition, arrestin binding to CXCR4 is driven by this phosphorylation of far C-terminal residues. Thus, site-specific phosphorylationof CXCR4 may be regulated by multiple kinases lead to complex regulation of CXCR4 signaling.
Background: The chemokine receptor CXCR4 is a widely expressed G protein-coupled receptor required for development, hematopoiesis, organogenesis, and vascularization. In disease, CXCR4 has been implicated in WHIM syndrome, HIV, and cancers. Regulation of CXCR4 function occurs through phosphorylation at multiple sites in the C-terminal region. These sites have been shown to be phosphorylated after CXCL12 stimulation, and involve several kinases, such as PKCand GRK kinases. After CXCL12 stimulation of HEK293 cells, Ser-324 and Ser-325 become phosphorylated by PKC andGRK6, while Ser-330 and Ser-339 are phosphorylated by only GRK6. In human astroglia cells, Ser-324 and Ser-325 are rapidly phosphorylated in endogenous CXCR4, while Ser-330 was phosphorylated with slower kinetics. In addition, arrestin binding to CXCR4 is driven by this phosphorylation of far C-terminal residues. Thus, site-specific phosphorylationof CXCR4 may be regulated by multiple kinases lead to complex regulation of CXCR4 signaling.
Similar Products
Product Notes
The Human, Rat, Mouse, Chicken CXCR4 (Catalog #AAA474211) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474211 Antibody Sampler Kit recognizes Human, Rat, Mouse, Chicken CXCR4. AAA Biotech's CXCR4 Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the CXCR4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCR4 Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
