CREB Phospho-Regulation Antibody Sampler Kit | CREB antibody sampler kit
CREB Phospho-Regulation
Reactivity
Human, Rat, Mouse, Chicken
Applications
Western Blot, ELISA
Synonyms
CREB Phospho-Regulation; CREB antibody sampler kit
Reactivity
Human, Rat, Mouse, Chicken
Form/Format
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for CREB antibody sampler kit
Western Blot (WB), ELISA (EIA)
Preparation and Storage
Store all at -20 degree C.Stable for 1 year.
Related Product Information for CREB antibody sampler kit
Kit Summary: The CREB phospho-regulation antibody sampler kit can be used to examine Ser-133 and Ser-271 phosphorylation inCREB. The kit also includes a rabbit polyclonal antibody to monitor total expression levels of CREB, and HRP-conjugatedsecondary reagents.
Background: CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles incell survival and proliferation. CREB is expressed in various tissues, and has important gene-regulating roles in thenervous system. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases. CREB is phosphorylated at other sites in response to calciuminflux and DNA damage. The DNA-damage responsive nuclear kinase, HIPK2, can phosphorylate Ser-271 but not Ser-133 in CREB, and this phosphorylation activates CREB transactivation function. Mutation of Ser-271 to Glu-271potentiates the CREB transactivation function. Thus, phosphorylation of Ser-271 may be the mode of activation forCREB-dependent transcription in response to genotoxic stress.
Background: CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles incell survival and proliferation. CREB is expressed in various tissues, and has important gene-regulating roles in thenervous system. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases. CREB is phosphorylated at other sites in response to calciuminflux and DNA damage. The DNA-damage responsive nuclear kinase, HIPK2, can phosphorylate Ser-271 but not Ser-133 in CREB, and this phosphorylation activates CREB transactivation function. Mutation of Ser-271 to Glu-271potentiates the CREB transactivation function. Thus, phosphorylation of Ser-271 may be the mode of activation forCREB-dependent transcription in response to genotoxic stress.
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Product Notes
The Human, Rat, Mouse, Chicken CREB (Catalog #AAA474210) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474210 Antibody Sampler Kit recognizes Human, Rat, Mouse, Chicken CREB. AAA Biotech's CREB Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the CREB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CREB Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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