Typical Testing Data/Standard Curve (for reference only)
Chicken TP ELISA Kit | TP elisa kit
Chicken TP (Thymidine Phosphorylase) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TP. You can calculate the concentration of TP in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, May 2010]
Uniprot Description
TMPO: a single-pass type II membrane protein. Tightly associated with the nuclear lamina. Binds DNA and involved in DNA replication and chromatin remodeling, May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with HAP95. Associated with dilated cardiomyopathy; upregulated in medulloblastoma. Used as a pharmaceutical to treat primary and secondary immune deficiencies, autoimmunity, infections and cancer. Available under the names Timunox (Cilag), Sintomodulina (Italofarmaco) and Mepentil (Recordati). Three alternatively spliced isoforms have been described.
Protein type: Membrane protein, integral; DNA-binding
Chromosomal Location of Human Ortholog: 12q22
Cellular Component: nuclear membrane; membrane; cytoplasm; integral to membrane; nuclear inner membrane; nuclear envelope; chromatin; nucleus
Molecular Function: protein binding; DNA binding; lamin binding
Biological Process: regulation of transcription, DNA-dependent
Research Articles on TP
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Product Notes
The Chicken TP tmpo (Catalog #AAA2508682) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2508682 ELISA Kit recognizes Chicken TP. It is sometimes possible for the material contained within the vial of "TP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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