Chicken Protein artemis (DCLRE1C) ELISA Kit | DCLRE1C elisa kit

Chicken Protein artemis (DCLRE1C) ELISA Kit

Cat. Num. AAA2800162

• Gene Names: DCLRE1C; SNM1C; artemis; nuclease
• Reactivity: Chicken
• Synonyms: Protein artemis (DCLRE1C); Chicken Protein artemis (DCLRE1C) ELISA Kit; RP11-398C13.5; A-SCID; DCLREC1C; FLJ11360; FLJ36438; RS-SCID; SCIDA; SNM1C; OTTHUMP00000019166; OTTHUMP00000019167; OTTHUMP00000019168; OTTHUMP00000019170; OTTHUMP00000019171; OTTHUMP00000019172; artemis; DCLRE1C elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chicken DCLRE1C. No significant cross-reactivity or interference between Chicken DCLRE1C and analogues was observed.
• Sequence Length: 714

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DCLRE1C elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate DCLRE1C in samples. An antibody specific for DCLRE1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DCLRE1C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCLRE1C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCLRE1C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: DCLRE1C encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins when complexed with protein kinase, DNA-activated, catalytic polypeptide.The purified Artemis protein alone possesses single-strand-specific 5-prime-to-3-prime exonuclease activity. Upon complex formation, PRKDC phosphorylates Artemis, and Artemis acquires endonucleolytic activity on 5-prime and 3-prime overhangs, as well as hairpins. PRKDC regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5-prime and 3-prime overhang processing in nonhomologous DNA end joining.

NCBI and Uniprot Product Information

• NCBI GI #: 71896183
• NCBI GeneID: 430764
• NCBI Accession #: NP_001026765.1
• NCBI GenBank Nucleotide #: NM_001031594.1
• UniProt Accession #: Q5QJC2
• Molecular Weight: 80,677 Da
• NCBI Official Full Name: protein artemis
• NCBI Official Synonym Full Names: DNA cross-link repair 1C
• NCBI Official Symbol: DCLRE1C
• NCBI Official Synonym Symbols: SNM1C; artemis; nuclease
• NCBI Protein Information: protein artemis
• UniProt Protein Name: Protein artemis
• Protein Family: Protein artemis
• UniProt Gene Name: DCLRE1C
• UniProt Synonym Gene Names: SNM1C; chSNM1C

Uniprot Description

Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation, and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ ().