Chicken PMP3 ELISA Kit | PMP3 elisa kit
Chicken PMP3 (Peroxisomal Membrane Protein 3) ELISA Kit
Reactivity
Chicken
Synonyms
PMP3; Chicken PMP3 (Peroxisomal Membrane Protein 3) ELISA Kit; PMP3 elisa kit
Reactivity
Chicken
Specificity
This kit recognizes natural and recombinant Chicken PMP3. No significant cross-reactivity or interference between Chicken PMP3 and analogues was observed.
Sequence Length
57
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PMP3 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken PMP3 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PMP3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PMP3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PMP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PMP3. You can calculate the concentration of PMP3 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PMP3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PMP3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PMP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PMP3. You can calculate the concentration of PMP3 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
6,179 Da
NCBI Official Full Name
plasma membrane proteolipid Pmp3
NCBI Official Symbol
pmp3
NCBI Protein Information
plasma membrane proteolipid Pmp3
UniProt Protein Name
Plasma membrane proteolipid 3
Protein Family
UniProt Gene Name
pmp3
UniProt Entry Name
PMP3_SCHPO
Uniprot Description
Plays a role in the regulation of membrane potential. Could mediate a proton leak. Regulated by the spc1-atf1 pathway and so expression is stress-induced.
