Typical Testing Data/Standard Curve (for reference only)
Chicken Interferon alpha/beta receptor 1 (IFNAR1) ELISA Kit | IFNAR1 elisa kit
Chicken Interferon alpha/beta receptor 1 (IFNAR1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: IFNalpha/betaR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IFNalpha/betaR1 antibody and an IFNalpha/ betaR1-HRP conjugate. The assay sample and buffer are incubated together with IFNalpha/betaR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNalpha/betaR1 concentration since IFNalpha/betaR1 from samples and IFN alpha/betaR1-HRP conjugate compete for the anti-IFNalpha/betaR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNalpha/betaR1 from the sample, fewer sites are left to bind IFN alpha/betaR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNalpha/betaR1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq, Jul 2008]
Uniprot Description
IFNAR1: receptor for interferons alpha and beta. Belongs to the type II cytokine family of receptors. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. Tyk2 tyrosine kinase is essential for stable cell surface expression of IFNAR1. Present in all tissues and even on the surface of most IFN-resistant cells.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 21q22.11
Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane
Molecular Function: interferon receptor activity; protein binding; interferon-alpha/beta receptor activity
Biological Process: positive regulation of interferon-gamma production; cell surface receptor linked signal transduction; T cell activation; cytokine and chemokine mediated signaling pathway; response to virus; positive regulation of interleukin-1 beta secretion; JAK-STAT cascade; interferon type I biosynthetic process; defense response to virus
Research Articles on IFNAR1
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Product Notes
The Chicken IFNAR1 ifnar1 (Catalog #AAA7231513) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7231513 ELISA Kit recognizes Chicken IFNAR1. It is sometimes possible for the material contained within the vial of "Interferon alpha/beta receptor 1 (IFNAR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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