Chicken Gamma secretase ELISA Kit | Gamma secretase elisa kit

Chicken Gamma secretase ELISA Kit

Cat. Num. AAA755855

• Gene Names: GBE1; GBE; APBD; GSD4
• Reactivity: Chicken
• Synonyms: Gamma secretase; Chicken Gamma secretase ELISA Kit; Gamma secretase elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This gamma Secretase ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken gamma Secretase. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This gamma Secretase ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken gamma Secretase. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Gamma secretase elisa kit

Principle of the Assay: gamma Secretase ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-gamma Secretase antibody and an gamma Secretase-HRP conjugate. The assay sample and buffer are incubated together with gamma Secretase-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the gamma Secretase concentration since gamma Secretase from samples and gamma Secretase-HRP conjugate compete for the anti-gamma Secretase antibody binding site. Since the number of sites is limited, as more sites are occupied by gamma Secretase from the sample, fewer sites are left to bind gamma Secretase-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The gamma Secretase concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Gamma secretase elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 119589286
• NCBI GeneID: 2632
• Molecular Weight: 80,474 Da
• NCBI Official Full Name: glucan (1,4-alpha-), branching enzyme 1 (glycogen branching enzyme, Andersen disease, glycogen storage disease type IV), isoform CRA_b
• NCBI Official Synonym Full Names: glucan (1,4-alpha-), branching enzyme 1
• NCBI Official Symbol: GBE1
• NCBI Official Synonym Symbols: GBE; APBD; GSD4
• NCBI Protein Information: 1,4-alpha-glucan-branching enzyme; brancher enzyme; glycogen branching enzyme; glycogen-branching enzyme; amylo-(1,4 to 1,6) transglucosidase; amylo-(1,4 to 1,6) transglycosylase
• UniProt Protein Name: 1,4-alpha-glucan-branching enzyme
• UniProt Gene Name: GBE1
• UniProt Entry Name: GLGB_HUMAN

NCBI Description

The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq, Jul 2008]

Uniprot Description

GBE1: a monomeric enzyme that functions in glycogen symthesis by catalyzing the formation of alpha 1,6- glucosidic linkages.Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells. It is most highly expressed in liver and muscle. Deficiency can result in glycogen storage disease IV (Andersen's disease).

Protein type: EC 2.4.1.18; Transferase; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 3p12.3

Cellular Component: cytosol

Molecular Function: cation binding; 1,4-alpha-glucan branching enzyme activity; hydrolase activity, hydrolyzing O-glycosyl compounds

Biological Process: glycogen metabolic process; generation of precursor metabolites and energy; glycogen biosynthetic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis

Disease: Polyglucosan Body Disease, Adult Form; Glycogen Storage Disease Iv