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Typical Testing Data/Standard Curve

Chicken Endothelin 1 (ET-1) ELISA Kit | ET-1 elisa kit

Chicken Endothelin 1 (ET-1) ELISA Kit

Gene Names
EDN1; ET1; QME; PPET1; ARCND3; HDLCQ7
Reactivity
Chicken
Synonyms
Endothelin 1 (ET-1); Chicken Endothelin 1 (ET-1) ELISA Kit; ET-1 elisa kit
Ordering
For Research Use Only!
Reactivity
Chicken
Specificity
No cross-reaction with other factors.
Sequence Length
212
Samples
Chicken serum, plasma or cell culture supernatant and organizations in the natural and recombinant ET-1 concentration.
Assay Type
Sandwich
Detection Range
1000 pg/ml-15.6pg/ml
Sensitivity
5pg/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Typical Testing Data/Standard Curve
Related Product Information for ET-1 elisa kit
Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken ET-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
24,425 Da
NCBI Official Full Name
endothelin-1
NCBI Official Synonym Full Names
endothelin 1
NCBI Official Symbol
EDN1
NCBI Official Synonym Symbols
ET1; QME; PPET1; ARCND3; HDLCQ7
NCBI Protein Information
endothelin-1; preproendothelin-1
UniProt Protein Name
Endothelin-1
UniProt Gene Name
EDN1
UniProt Synonym Gene Names
PPET1; ET-1
UniProt Entry Name
EDN1_HUMAN

NCBI Description

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

EDN1: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p24.1

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: protein binding; hormone activity; endothelin B receptor binding; cytokine activity; endothelin A receptor binding

Biological Process: response to nicotine; positive regulation of JNK activity; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of nitric oxide biosynthetic process; regulation of systemic arterial blood pressure by endothelin; heart development; response to lipopolysaccharide; middle ear morphogenesis; prostaglandin biosynthetic process; sensory perception of pain; positive regulation of MAP kinase activity; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; cell surface receptor linked signal transduction; cell-cell signaling; negative regulation of nitric-oxide synthase biosynthetic process; protein kinase C activation; cell growth; neutrophil chemotaxis; rhythmic excitation; positive regulation of mitosis; negative regulation of blood coagulation; positive regulation of heart rate; response to testosterone stimulus; respiratory gaseous exchange; peptide hormone secretion; response to amino acid stimulus; leukocyte activation; patterning of blood vessels; membrane depolarization; protein kinase C deactivation; regulation of vasoconstriction; positive regulation of transcription from RNA polymerase II promoter; response to activity; superoxide release; positive regulation of odontogenesis; epithelial fluid transport; phosphoinositide 3-kinase cascade; positive regulation of cell size; neural crest cell development; G-protein signaling, phospholipase D activating pathway; negative regulation of hormone secretion; positive regulation of smooth muscle cell proliferation; negative regulation of cellular protein metabolic process; negative regulation of transcription from RNA polymerase II promoter; glucose transport; vein smooth muscle contraction; histamine secretion; nitric oxide transport; positive regulation of cell proliferation; regulation of pH; positive regulation of smooth muscle contraction; artery smooth muscle contraction; vasoconstriction; inositol phosphate-mediated signaling; in utero embryonic development; positive regulation of hormone secretion; calcium-mediated signaling; multicellular organismal aging; body fluid secretion; G-protein coupled receptor protein signaling pathway; dorsal/ventral pattern formation; response to ozone; cartilage development; positive regulation of prostaglandin secretion; maternal process involved in parturition; regulation of sensory perception of pain; positive regulation of cell migration

Disease: Question Mark Ears, Isolated; Auriculocondylar Syndrome 3

Research Articles on ET-1

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Product Notes

The Chicken ET-1 edn1 (Catalog #AAA261706) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261706 ELISA Kit recognizes Chicken ET-1. It is sometimes possible for the material contained within the vial of "Endothelin 1 (ET-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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