Chicken CHRNA7-FAM7A fusion protein (CHRFAM7A) ELISA Kit | CHRFAM7A elisa kit

Chicken CHRNA7-FAM7A fusion protein (CHRFAM7A) ELISA Kit

Cat. Num. AAA7206408

• Gene Names: CHRFAM7A; D-10; CHRNA7; CHRNA7-DR1
• Reactivity: Chicken
• Synonyms: CHRNA7-FAM7A fusion protein (CHRFAM7A); Chicken CHRNA7-FAM7A fusion protein (CHRFAM7A) ELISA Kit; CHRFAM7A elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This CHRFAM7A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CHRFAM7A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRFAM7A elisa kit

Principle of the assay: CHRFAM7A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CHRFAM7A antibody and an CHRFAM7A-HRP conjugate. The assay sample and buffer are incubated together with CHRFAM7A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHRFAM7A concentration since CHRFAM7A from samples and CHRFAM7A-HRP conjugate compete for the anti-CHRFAM7A antibody binding site. Since the number of sites is limited, as more sites are occupied by CHRFAM7A from the sample, fewer sites are left to bind CHRFAM7A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHRFAM7A concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 23312390
• NCBI GeneID: 89832
• NCBI Accession #: NP_683709.1
• NCBI GenBank Nucleotide #: NM_148911.1
• Molecular Weight: 46,218 Da
• NCBI Official Full Name: CHRNA7-FAM7A fusion protein isoform 2
• NCBI Official Synonym Full Names: CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion
• NCBI Official Symbol: CHRFAM7A
• NCBI Official Synonym Symbols: D-10; CHRNA7; CHRNA7-DR1
• NCBI Protein Information: CHRNA7-FAM7A fusion protein; alpha-7 nicotinic cholinergic receptor subunit; alpha 7 neuronal nicotinic acetylcholine receptor-FAM7A hybrid; CHRNA7 (cholinergic receptor, nicotinic, alpha polypeptide 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion
• UniProt Protein Name: CHRNA7-FAM7A fusion protein
• Protein Family: CHRNA7-FAM7A fusion protein
• UniProt Gene Name: CHRFAM7A
• UniProt Entry Name: CRFM7_HUMAN

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The family member CHRNA7, which is located on chromosome 15 in a region associated with several neuropsychiatric disorders, is partially duplicated and forms a hybrid with a novel gene from the family with sequence similarity 7 (FAM7A). Alternative splicing has been observed, and two variants exist, for this hybrid gene. The N-terminally truncated products predicted by the largest open reading frames for each variant would lack the majority of the neurotransmitter-gated ion-channel ligand binding domain but retain the transmembrane region that forms the ion channel. Although current evidence supports transcription of this hybrid gene, translation of the nicotinic acetylcholine receptor-like protein-encoding open reading frames has not been confirmed. [provided by RefSeq, Jul 2008]

Uniprot Description

CHRFAM7A: Belongs to the ligand-gated ion channel (TC 1.A.9) family

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q13.1

Cellular Component: integral to membrane

Molecular Function: extracellular ligand-gated ion channel activity