Typical Testing Data/Standard Curve (for reference only)
Chicken ADCY4 ELISA Kit | ADCY4 elisa kit
Chicken ADCY4 (Adenylate Cyclase 4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADCY4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADCY4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADCY4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ADCY4. You can calculate the concentration of ADCY4 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). Mouse studies show that adenylate cyclase 4, along with adenylate cyclases 2 and 3, is expressed in olfactory cilia, suggesting that several different adenylate cyclases may couple to olfactory receptors and that there may be multiple receptor-mediated mechanisms for the generation of cAMP signals. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010]
Uniprot Description
ADCY4: This is a membrane-bound, calmodulin-insensitive adenylyl cyclase. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Protein type: Lyase; EC 4.6.1.1; Membrane protein, multi-pass; Nucleotide Metabolism - purine; Transporter, aquaporin family; Membrane protein, integral; Transporter; Adenylyl cyclase
Chromosomal Location of Human Ortholog: 14q12
Cellular Component: membrane; cytoplasm; dendrite; integral to membrane; plasma membrane
Molecular Function: protein binding; metal ion binding; ATP binding; adenylate cyclase activity
Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; epidermal growth factor receptor signaling pathway; synaptic transmission; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; phospholipase C activation; water transport; activation of protein kinase A; energy reserve metabolic process; innate immune response; renal water homeostasis; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; signal transduction; transmembrane transport; G-protein signaling, adenylate cyclase activating pathway
Research Articles on ADCY4
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Product Notes
The Chicken ADCY4 adcy4 (Catalog #AAA2502904) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2502904 ELISA Kit recognizes Chicken ADCY4. It is sometimes possible for the material contained within the vial of "ADCY4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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