Ovalbumin Protein
Ovalbumin (Agarose)
Purity
Molecular Biology Grade
Synonyms
Ovalbumin; Ovalbumin (Agarose); Ovalbumin protein
Host
Chicken egg ovalbumin
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a suspension in PBS, pH 7.2, sodium azide.
Application Notes
Suitable for use in antibody purification.
Dilution: Regeneration:
Wash with 3ml of 0.1M glycine buffer, pH 2.5, and then immediately washing with PBS pH 7.4 with 10-20 volumes. Store column in PBS containing 0.05% azide at 4 degree C. DO NOT FREEZE the beads at any stage.Optimal dilutions to be determined by the researcher.
Dilution: Regeneration:
Wash with 3ml of 0.1M glycine buffer, pH 2.5, and then immediately washing with PBS pH 7.4 with 10-20 volumes. Store column in PBS containing 0.05% azide at 4 degree C. DO NOT FREEZE the beads at any stage.Optimal dilutions to be determined by the researcher.
Preparation and Storage
Stored at 4 degree C. Do not freeze. Aliquots are stable for at least 6 months from date of opening.
Related Product Information for Ovalbumin protein
Ovalbumin is a glycoprotein and the major protein of egg white. It has a molecular weight of 43000. Ovalbumin is used for conjugation or immunological determinations as well as drug and pharmaceutical processing.
Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, DYKDDDDK-epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. Many tags have their own characteristics. Poly-His-fusion proteins (6x His) can bind to Nickel-Sepharose or Nickel-HRP. GST-fusion proteins can bind to glutathione-Sepharose. Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.
Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, DYKDDDDK-epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. Many tags have their own characteristics. Poly-His-fusion proteins (6x His) can bind to Nickel-Sepharose or Nickel-HRP. GST-fusion proteins can bind to glutathione-Sepharose. Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.
Product Categories/Family for Ovalbumin protein
NCBI and Uniprot Product Information
Uniprot Description
ovalbumin: Non-inhibitory serpin. Storage protein of egg white. Belongs to the serpin family. Ov-serpin subfamily.
Cellular Component: extracellular space; extracellular region; phagocytic vesicle; cytosol
Molecular Function: serine-type endopeptidase inhibitor activity; calcium ion binding
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Product Notes
The Ovalbumin (Catalog #AAA634830) is a Protein produced from Chicken egg ovalbumin and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in antibody purification. Dilution: Regeneration: Wash with 3ml of 0.1M glycine buffer, pH 2.5, and then immediately washing with PBS pH 7.4 with 10-20 volumes. Store column in PBS containing 0.05% azide at 4 degree C. DO NOT FREEZE the beads at any stage.Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Ovalbumin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ovalbumin, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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