Human Chitinase-3-like protein 1 ELISA Kit | CHI3L1 elisa kit

Human Chitinase-3-like protein 1 ELISA Kit

Cat. Num. AAA765293

• Gene Names: CHI3L1; GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
• Reactivity: Human
• Synonyms: Chitinase-3-like protein 1; Human Chitinase-3-like protein 1 ELISA Kit; CHI3L1 (Chitinase-3-like protein 1)/GP39/Glycoprotein 39; Cartilage/YKL-40/ASRT7/CGP-39/HC-gp39/YYL-40; CHI3L1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CHI3L1. No significant cross-reactivity or interference between CHI3L1 and analogues was observed.
• Sequence Length: 383

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHI3L1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 144226251
• NCBI GeneID: 1116
• NCBI Accession #: NP_001267.2
• NCBI GenBank Nucleotide #: NM_001276.2
• Molecular Weight: 42,625 Da
• NCBI Official Full Name: chitinase-3-like protein 1
• NCBI Official Synonym Full Names: chitinase 3 like 1
• NCBI Official Symbol: CHI3L1
• NCBI Official Synonym Symbols: GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
• NCBI Protein Information: chitinase-3-like protein 1
• UniProt Protein Name: Chitinase-3-like protein 1
• Protein Family: Chitinase-3-like protein
• UniProt Gene Name: CHI3L1
• UniProt Synonym Gene Names: CGP-39; GP-39; hCGP-39
• UniProt Entry Name: CH3L1_HUMAN

NCBI Description

Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]

Uniprot Description

CHI3L1: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Belongs to the glycosyl hydrolase 18 family.

Protein type: Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: cytoplasm; endoplasmic reticulum; extracellular space; perinuclear region of cytoplasm; proteinaceous extracellular matrix

Molecular Function: carbohydrate binding; chitin binding; chitinase activity; extracellular matrix structural constituent

Biological Process: activation of NF-kappaB-inducing kinase; apoptosis; carbohydrate metabolic process; cartilage development; chitin catabolic process; inflammatory response; lung development; positive regulation of angiogenesis; positive regulation of protein kinase B signaling cascade; response to mechanical stimulus

Disease: Asthma-related Traits, Susceptibility To, 7; Schizophrenia