Chromogranin A Recombinant Protein | CHGA recombinant protein

Recombinant Chromogranin A (CHGA)

Cat. Num. AAA2028861

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Chromogranin A; Recombinant Chromogranin A (CHGA); CHGA recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; LPVN SPMNKGDTEV MKCIVEVISD TLSKPSPMPVSQECFETLRG DERILSILRH QNLLKELQDL ALQGAKERSH QQKKQSSYEDELSEVLEKQN DQAELKEGTE EASSKEAAEK RGDSKEVEKN DEDADGAKPQASLEPPXXXE AEDQTPGEEE AASTHPLASL PSKKRPGAQA EEDHEGPSQGPVDREKGPSA EQGPQAEREE EEEAEAGEKA VPEEEGPRSE AFDSHPSLGYKEMQRGWPQA PAMDGAGKTG AEEAQPPEGK GAREHSRQEE EEETAGAPQGLFRGGKRGEP AQEEEERLSE EWENAKRWSK MDRLAKELTA EKRLQGEEEEEEEEEDPDRS MKLSFRAPAY GFRGPGLQLR RGWRPSSRED SVEAGLPLQVRXYLEEKKEE EGSANRRPED QELESLSAIE AELEKVAPQL QSLRRG
• Sequence Length: 457

• Applicable Applications for CHGA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 51.0KDa
• Accurate Molecular Mass (KD): 52kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Sus scrofa; Porcine (Pig)
• Fragment: Leu17~Gly446
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 180527
• Molecular Weight: 50,688 Da
• NCBI Official Full Name: chromogranin A
• UniProt Protein Name: Chromogranin-A
• Protein Family: Chromogranin
• UniProt Gene Name: CHGA
• UniProt Synonym Gene Names: CgA; SP-I
• UniProt Entry Name: CMGA_HUMAN

Uniprot Description

CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells.

Protein type: Vesicle; Cell adhesion; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: perinuclear region of cytoplasm; extracellular region; secretory granule

Biological Process: regulation of blood pressure