Human complement factor H ELISA Kit | CFH elisa kit

Human complement factor H, CFH ELISA Kit

Cat. Num. AAA701752

• Gene Names: CFH; FH; HF; HF1; HF2; HUS; FHL1; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3
• Reactivity: Human
• Synonyms: complement factor H; Human complement factor H; CFH ELISA Kit; AHUS1; ARMD4; ARMS1; CFHL3; FH; FHL1; HF; HF1; HF2; HUS; MGC88246; H factor 1 (complement) ; H factor 2 (complement) ; age-related maculopathy susceptibility 1; beta-1-H-globulin; beta-1H; complement facto; CFH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CFH. No significant cross-reactivity or interference between human CFH and analogues was observed.
• Sequence Length: 1231

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2 ng/ml-2000 ng/ml
• Sensitivity: < 7.8 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFH elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CFH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CFH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CFH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CFH bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 62739186
• NCBI GeneID: 3075
• NCBI Accession #: NP_000177.2
• NCBI GenBank Nucleotide #: NM_000186.3
• UniProt Accession #: P08603; P78435; Q14570; Q2TAZ5; Q38G77; Q5TFM3; Q8N708; Q9NU86; A5PL14
• Molecular Weight: 139,096 Da
• NCBI Official Full Name: complement factor H isoform a
• NCBI Official Synonym Full Names: complement factor H
• NCBI Official Symbol: CFH
• NCBI Official Synonym Symbols: FH; HF; HF1; HF2; HUS; FHL1; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3
• NCBI Protein Information: complement factor H; beta-1H; factor H-like 1; beta-1-H-globulin; H factor 1 (complement); H factor 2 (complement); adrenomedullin binding protein; complement factor H, isoform b; age-related maculopathy susceptibility 1
• UniProt Protein Name: Complement factor H
• Protein Family: Complement factor
• UniProt Gene Name: CFH
• UniProt Synonym Gene Names: HF; HF1; HF2
• UniProt Entry Name: CFAH_HUMAN

NCBI Description

This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Oct 2011]

Uniprot Description

Function: Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.