Human Complement Factor D ELISA Kit | CFD elisa kit

Complement Factor D (Human) OmniKine ELISA Kit

Cat. Num. AAA9502038

• Gene Names: CFD; DF; ADN; PFD; ADIPSIN
• Reactivity: Human
• Synonyms: Complement Factor D; Complement Factor D (Human) OmniKine ELISA Kit; Adipsin; C3 convertase activator; Properdin factor D; CFD elisa kit

• Reactivity: Human
• Specificity: The OmniKine Human Complement Factor D ELISA is capable of recognizing both recombinant and naturally produced Human Complement Factor D proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.; Human: Coagulation Factor II, Coagulation Factor X, Coagulation Factor XI
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 260

• Samples: Plasma, serum, lysate
• Assay Type: Sandwich ELISA
• Detection Range: 32-2000 pg/ml
• Sensitivity: 32-2000 pg/ml
• Detection Method: Colorimetric 450 nm
• Subtype: None
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human Complement Factor D ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Complement Factor D proteins within the range of 32-2000 pg/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 955654851
• NCBI GeneID: 1675
• NCBI Accession #: NP_001304264.1
• NCBI GenBank Nucleotide #: NM_001317335.1
• UniProt Accession #: P00746; Q5U5S1; Q86VJ5; Q8N4E0; Q8WZB4; B4DV76
• Molecular Weight: 27,033 Da
• NCBI Official Full Name: complement factor D isoform 2
• NCBI Official Synonym Full Names: complement factor D
• NCBI Official Symbol: CFD
• NCBI Official Synonym Symbols: DF; ADN; PFD; ADIPSIN
• NCBI Protein Information: complement factor D
• UniProt Protein Name: Complement factor D
• Protein Family: Complement factor
• UniProt Gene Name: CFD
• UniProt Synonym Gene Names: DF; PFD

NCBI Description

This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease. [provided by RefSeq, Nov 2015]

Uniprot Description

CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.46; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: complement activation; complement activation, alternative pathway; neutrophil degranulation; platelet degranulation; proteolysis

Disease: Complement Factor D Deficiency