Carboxylesterase 1 Recombinant Protein | CES1 recombinant protein

Recombinant Carboxylesterase 1 (CES1)

Cat. Num. AAA2033057

• Gene Names: Ces1c; Ee1; Es1; Es4; EsN; Ee-1; Es-4; Es-N; PESN; Ces-N
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Carboxylesterase 1; Recombinant Carboxylesterase 1 (CES1); ACAT; CEH; CES2; HMSE1; PCE1; SES1; TGH; Retinyl ester hydrolase; Serine esterase 1; Triacylglycerol hydrolase; Egasyn; Cocaine carboxylesterase; Liver Carboxylesterase 1; CES1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: GHP SSPPVVDTVH GKVLGKFVSL EGFAQPVAIFLGIPFAKPPL GPLRFTPPQP AEPWSFVKNA TSYPPMCTQD PKAGQLLSELFTNRKENIPL KLSEDCLYLN IYTPADLTKK NRLPVMVWIH GGGLMVGAASTYDGLALAAH ENVVVVTIQY RLGIWGFFST GDEHSRGNWG HLDQVAALRWVQDNIASFGG NPGSVTIFGE SAGGESVSVL VLSPLAKNLF HRAISESGVALTSVLVKKGD VKPLAEQIAI TAGCKTTTSA VMVHCLRQKT EEELLETTL
• Sequence Length: 554

• Applicable Applications for CES1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 34.0kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Gly18~Leu299
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 192854
• NCBI GeneID: 13884
• Molecular Weight: 61,056 Da
• NCBI Official Full Name: carboxylesterase
• NCBI Official Synonym Full Names: carboxylesterase 1C
• NCBI Official Symbol: Ces1c
• NCBI Official Synonym Symbols: Ee1; Es1; Es4; EsN; Ee-1; Es-4; Es-N; PESN; Ces-N
• NCBI Protein Information: carboxylesterase 1C
• UniProt Protein Name: Carboxylesterase 1C
• Protein Family: Carboxylesterase
• UniProt Gene Name: Ces1c
• UniProt Synonym Gene Names: Es1
• UniProt Entry Name: EST1C_MOUSE

Uniprot Description

Es1: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Involved in the extracellular metabolism of lung surfactant. Belongs to the type-B carboxylesterase/lipase family.

Protein type: EC 3.1.1.1

Cellular Component: endoplasmic reticulum; extracellular space

Molecular Function: 1-oxa-2-oxocycloheptane lactonase activity; 3,4-dihydrocoumarin hydrolase activity; all-trans-retinyl-palmitate hydrolase activity; butyrolactone hydrolase activity; hydrolase activity; hydrolase activity, acting on ester bonds; lipase activity; phospholipase activity; prenylcysteine methylesterase activity; retinyl-palmitate esterase activity; sulfolactone hydrolase activity