CEP57 recombinant protein

CEP57 Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121816

• Gene Names: CEP57; MVA2; PIG8; TSP57
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CEP57; CEP57 Protein; Human; Recombinant (GST Tag); Human CEP57/MVA2 Protein (GST Tag); MVA2 Protein; PIG8 Protein; TSP57 Protein; centrosomal protein 57kDa; CEP57 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser118-Arg226

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human CEP57 (Q86XR8-1)(Ser118-Arg226) was expressed with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CEP57 recombinant protein

Background: CEP57 is a centrosomal protein and is involved in nucleating and stabilizing microtubules. CEP57 was initially identified as a regulator of centriole overduplication in an RNA interference screen. There is a link between altered microenvironmental signaling cues such as FGF-2 overexpression and mitotic instability and provide a rationale for the therapeutic targeting of the FGF-2/FGFR1/CEP57 axis in prostate cancer. CEP57 is involved in intracellular transport processes, and its overexpression causes mitotic defects as well as abnormal microtubule nucleation and bundling.

NCBI and Uniprot Product Information

• NCBI GI #: 344925823
• NCBI GeneID: 9702
• NCBI Accession #: NP_001230705.1
• NCBI GenBank Nucleotide #: NM_001243776.1
• UniProt Accession #: Q86XR8; Q14704; Q5JB46; Q8IXP0; Q9BVF9; A0PJH1; A8K5D0; B4DDP5
• Molecular Weight: 57,089 Da
• NCBI Official Full Name: centrosomal protein of 57 kDa isoform b
• NCBI Official Synonym Full Names: centrosomal protein 57kDa
• NCBI Official Symbol: CEP57
• NCBI Official Synonym Symbols: MVA2; PIG8; TSP57
• NCBI Protein Information: centrosomal protein of 57 kDa; translokin; FGF2-interacting protein; testis-specific protein 57; proliferation-inducing protein 8
• UniProt Protein Name: Centrosomal protein of 57 kDa
• Protein Family: Centrosomal protein
• UniProt Gene Name: CEP57
• UniProt Synonym Gene Names: KIAA0092; TSP57; Cep57
• UniProt Entry Name: CEP57_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein called Translokin. This protein localizes to the centrosome and has a function in microtubular stabilization. The N-terminal half of this protein is required for its centrosome localization and for its multimerization, and the C-terminal half is required for nucleating, bundling and anchoring microtubules to the centrosomes. This protein specifically interacts with fibroblast growth factor 2 (FGF2), sorting nexin 6, Ran-binding protein M and the kinesins KIF3A and KIF3B, and thus mediates the nuclear translocation and mitogenic activity of the FGF2. It also interacts with cyclin D1 and controls nucleocytoplasmic distribution of the cyclin D1 in quiescent cells. This protein is crucial for maintaining correct chromosomal number during cell division. Mutations in this gene cause mosaic variegated aneuploidy syndrome, a rare autosomal recessive disorder. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

CEP57: Centrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring- like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2. Defects in CEP57 are the cause of mosaic variegated aneuploidy syndrome type 2 (MVA2). MVA2 is a severe developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases. Belongs to the translokin family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nucleoplasm; microtubule cytoskeleton; Golgi apparatus; microtubule; centrosome; cytoplasm; nucleus; cytosol

Molecular Function: gamma-tubulin binding; protein binding; fibroblast growth factor binding; protein homodimerization activity; microtubule binding

Biological Process: fibroblast growth factor receptor signaling pathway; protein import into nucleus, translocation; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein homooligomerization; spermatid development

Disease: Mosaic Variegated Aneuploidy Syndrome 2