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Centromere Protein F Recombinant Protein | CENPF recombinant protein
Recombinant Centromere Protein F (CENPF)
Source: Prokaryotic expression
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QAELD KLTSVKQQLE NNLEEFKQKL CRAEQAFQAS QIKENELRRS MEEMKKENNL LKSHSEQKAR EVCHLEAELK NIKQCLNQSQ NFAEEMKAKN TSQETMLRDL QEKINQQENS LTLEKLKLAV ADLEKQRDCS QDLLKKREHH IEQLNDKLSK TEKESKALLS ALELKKKEYE ELKEEKTLFS CWKSENEKLLTQMESEK
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
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NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]
Uniprot Description
CENPF: a nuclear matrix protein that assembles onto kinetochores at late G2. Is hyperphosphorylated during mitosis and rapidly degraded after mitosis. Involved in chromosome segregation during mitosis. Interacts with retinoblastoma protein (RB), CENP-E and BUBR1. Two alternatively spliced human isoforms have been described.
Protein type: Transcription, coactivator/corepressor; Cell cycle regulation; Nuclear envelope
Chromosomal Location of Human Ortholog: 1q41
Cellular Component: spindle pole; nuclear matrix; pronucleus; nuclear envelope; cytosol; nucleoplasm; kinetochore; perinuclear region of cytoplasm; cytoplasm; outer kinetochore of condensed chromosome; spindle; midbody; nucleus; chromatin; chromosome, pericentric region
Molecular Function: protein C-terminus binding; dynein binding; protein binding; protein homodimerization activity; chromatin binding; transcription factor binding
Biological Process: response to drug; mitosis; muscle development; kinetochore assembly; regulation of cell cycle; chromosome segregation; cell proliferation; protein transport; cell division; mitotic cell cycle spindle assembly checkpoint; regulation of G2/M transition of mitotic cell cycle; metaphase plate congression; mitotic cell cycle; cell differentiation; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent; regulation of striated muscle development
Disease: Ciliary Dyskinesia, Primary, 31
Research Articles on CENPF
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Product Notes
The CENPF cenpf (Catalog #AAA2029482) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Centromere Protein F can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the CENPF cenpf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- QAELD KLTSVKQQLE NNLEEFKQKL CRAEQAFQAS QIKENELRRS MEEMKKENNL LKSHSEQKAR EVCHLEAELK NIKQCLNQSQ NFAEEMKAKN TSQETMLRDL QEKINQQENS LTLEKLKLAV ADLEKQRDCS QDLLKKREHH IEQLNDKLSK TEKESKALLS ALELKKKEYE ELKEEKTLFS CWKSENEKLL TQMESEK. It is sometimes possible for the material contained within the vial of "Centromere Protein F, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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