CENP-B Recombinant Protein | CENPB recombinant protein
CENP-B, Recombinant, Human (Centromere Protein B)
Purity
>95% (SDS-PAGE)
Synonyms
CENP-B; Recombinant; Human (Centromere Protein B); CENPB recombinant protein
Host
Sf9 insect cells
Purity/Purification
>95% (SDS-PAGE)
Form/Format
Supplied as a liquid in 20mM HEPES buffer, pH 7.6, 250mM sodium chloride and 20% glycerol.
Concentration
0.99mg/ml (varies by lot)
Application Notes
Suitable for use in ELISA, Western Blot, for biotinylation and iodination..
Grade
Highly Purified
Immunological Functions
1. Bins IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for CENPB recombinant protein
Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENP-B is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66kD and plays an important role in the formation of the centromeric chromatin. CENP-B antibodies are present in the sera of up to 80% of patients with CREST syndrome.
Recombinant Human CENP-B produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 80kD.
Recombinant Human CENP-B produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 80kD.
Product Categories/Family for CENPB recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
80kD
NCBI Official Full Name
CENP-B
UniProt Protein Name
Major centromere autoantigen B
Protein Family
UniProt Gene Name
CENPB
UniProt Synonym Gene Names
CENP-B
UniProt Entry Name
CENPB_HUMAN
Uniprot Description
CENPB: Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 20p13
Cellular Component: chromosome; nucleus; chromosome, pericentric region
Molecular Function: centromeric DNA binding; sequence-specific DNA binding; satellite DNA binding; chromatin binding
Biological Process: regulation of transcription, DNA-dependent
Similar Products
Product Notes
The CENP-B cenpb (Catalog #AAA638023) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in ELISA, Western Blot, for biotinylation and iodination.. Researchers should empirically determine the suitability of the CENP-B cenpb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENP-B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
