Human Centromere Protein A ELISA Kit | CENPA elisa kit

Human Centromere Protein A ELISA Kit

Cat. Num. AAA763783

• Gene Names: CENPA; CenH3; CENP-A
• Reactivity: Human
• Synonyms: Centromere Protein A; Human Centromere Protein A ELISA Kit; CENPA; CENPA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CENPA. No significant cross-reactivity or interference between CENPA and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between CENPA and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 114

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CENPA were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CENPA were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Related Product Information for CENPA elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CENPA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CENPA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CENPA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CENPA can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 12654133
• NCBI GeneID: 1058
• Molecular Weight: 13,001 Da
• NCBI Official Full Name: Centromere protein A
• NCBI Official Synonym Full Names: centromere protein A
• NCBI Official Symbol: CENPA
• NCBI Official Synonym Symbols: CenH3; CENP-A
• NCBI Protein Information: histone H3-like centromeric protein A
• UniProt Protein Name: Histone H3-like centromeric protein A
• Protein Family: Histone H3-like centromeric protein
• UniProt Gene Name: CENPA
• UniProt Synonym Gene Names: CENP-A
• UniProt Entry Name: CENPA_HUMAN

NCBI Description

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]

Uniprot Description

CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: chromosome, pericentric region; condensed nuclear chromosome, pericentric region; cytosol; inner kinetochore of condensed chromosome; nucleoplasm; nucleosome; nucleus

Molecular Function: chromatin binding; DNA binding; protein binding; protein heterodimerization activity

Biological Process: DNA replication-independent nucleosome assembly at centromere; establishment of mitotic spindle orientation; kinetochore assembly; mitotic cell cycle; nucleosome assembly; small GTPase mediated signal transduction; viral reproduction