Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) Recombinant Protein | CELSR2 recombinant protein

Recombinant Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2)

Cat. Num. AAA2009173

• Gene Names: CELSR2; EGFL2; MEGF3; CDHF10; Flamingo1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2); Recombinant Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2); CELSR2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- LAGVGHD FPFTINNGTG WISVAAELDR EEVDFYSFGV EARDHGTPAL TASASVSVTV LDVNDNNPTF TQPEYTVRLN EDAAVGTSVV TVSAVDRDAH SVITYQITSG NTRNRFSITS QSGGGLVSLA LPLDYKLERQ YVLAVTASDG TRQDTAQIVV NVTDANTHRP VFQSSHYTVN VNEDRPAGTT VVLISATDED TGENARITYF MEDSIPQFRI DADTGAVTTQ AELDYEDQVS YTLAITARDN GIPQKS
• Sequence Length: 2923

• Applicable Applications for CELSR2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Leu544~Ser796 (Accession # Q9HCU4) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13325064
• NCBI GeneID: 1952
• NCBI Accession #: NP_001399.1
• NCBI GenBank Nucleotide #: NM_001408.2
• UniProt Accession #: Q9HCU4; Q5T2Y7; Q92566
• Molecular Weight: 30.8kDa
• NCBI Official Full Name: cadherin EGF LAG seven-pass G-type receptor 2
• NCBI Official Synonym Full Names: cadherin, EGF LAG seven-pass G-type receptor 2
• NCBI Official Symbol: CELSR2
• NCBI Official Synonym Symbols: EGFL2; MEGF3; CDHF10; Flamingo1
• NCBI Protein Information: cadherin EGF LAG seven-pass G-type receptor 2; EGF-like protein 2; flamingo homolog 3; cadherin family member 10; EGF-like-domain, multiple 2; epidermal growth factor-like 2; multiple EGF-like domains protein 3; epidermal growth factor-like protein 2; multiple epidermal growth factor-like domains 3; multiple epidermal growth factor-like domains protein 3; cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)
• UniProt Protein Name: Cadherin EGF LAG seven-pass G-type receptor 2
• Protein Family: Cadherin EGF LAG seven-pass G-type receptor
• UniProt Gene Name: CELSR2
• UniProt Synonym Gene Names: CDHF10; EGFL2; KIAA0279; MEGF3; EGF-like protein 2
• UniProt Entry Name: CELR2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the flamingo subfamily, part of the cadherin superfamily. The flamingo subfamily consists of nonclassic-type cadherins; a subpopulation that does not interact with catenins. The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor-like repeats and two laminin A G-type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic unique to this subfamily. It is postulated that these proteins are receptors involved in contact-mediated communication, with cadherin domains acting as homophilic binding regions and the EGF-like domains involved in cell adhesion and receptor-ligand interactions. The specific function of this particular member has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CELSR2: Receptor that may have an important role in cell/cell signaling during nervous system formation. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 2; Membrane protein, integral; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: cytoplasm; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cerebrospinal fluid secretion; Wnt receptor signaling pathway; regulation of protein localization; regulation of cell-cell adhesion; regulation of transcription, DNA-dependent; neural plate anterioposterior pattern formation; ventricular system development; dendrite morphogenesis; neuron migration; cilium biogenesis; homophilic cell adhesion