USP8 cdna clone
USP8 cDNA Clone
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Uniprot Description
USP8: Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Forms a ternary complex with RNF128 and OTUB1. Interacts (via C-terminal UCH catalytic domain) with OTUB1 isoform 1. Interacts with STAM2 (via SH3 domain). Interacts with DNAJB3, EGFR, EPS15, RASGRF1, RNF41, YWHAE, YWHAG and YWHAZ. Interacts with NBR1, RASGRF1, RNF41 and IST1. Associates with the ESCRT-0 complex and with microtubules. Interacts with BIRC6/bruce and KIF23/MKLP1. Upon growth stimulation in starved human fibroblasts. Decreases in response to growth arrest induced by cell-cell contact. Belongs to the peptidase C19 family.
Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease
Chromosomal Location of Human Ortholog: 15q21.2
Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; early endosome; extrinsic to plasma membrane; Golgi apparatus; midbody; nucleoplasm
Molecular Function: cysteine-type endopeptidase activity; protein binding; ubiquitin-specific protease activity
Biological Process: cell proliferation; cytokinesis after mitosis; endosome organization and biogenesis; protein deubiquitination
Research Articles on USP8
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Product Notes
The USP8 usp8 (Catalog #AAA1273357) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: ATGCCTGCTG TGGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT AAAAGACCTT AATAAGAAGA CAGAAGTTAA ACCAGAGAAA ATAAGCACTA AGAGTTATGT GCACAGTGCC CTGAAGATCT TTAAGACAGC AGAAGAATGC AGATTAGATC GTGATGAGGA AAGGGCCTAT GTACTATATA TGAAATACGT GACTGTTTAT AATCTTATCA AAAAAAGACC TGATTTCAAG CAACAGCAGG ATTATTTCCA TTCAATACTT GGACCTGGAA ACATCAAAAA AGCTGTCGAA GAAGCTGAAA GACTCTCTGA AAGCCTTAAA TTAAGATATG AAGAAGCTGA AGTCCGGAAA AAACTTGAGG AAAAAGACAG GCAGGAGGAA GCACAGCGGC TACAACAAAA AAGGCAGGAA ACAGGAAGAG AGGATGGTGG CACATTGGCT AAAGGCTCTT TGGAGAATGT TTTGGATTCC AAAGACAAAA CCCAAAAGAG CAATGGTGAA AAGAATGAAA AATGTGAGAC CAAAGAGAAA GGAGCAATCA CAGCAAAGGA ACTATACACA ATGATGACGG ATAAAAACAT CAGCTTGATT ATAATGGATG CTCGAAGAAT GCAGGATTAT CAGGATTCCT GTATTTTACA TTCTCTCAGT GTTCCTGAAG AAGCCATCAG TCCAGGAGTC ACTGCTAGTT GGATTGAAGC ACACCTGCCA GATGATTCTA AAGACACATG GAAGAAGAGG GGGAATGTGG AGTATGTGGT ACTTCTTGAC TGGTTTAGTT CTGCCAAAGA TTTACAGATT GGAACAACTC TCCGGAGTCT GAAAGATGCA CTTTTCAAGT GGGAAAGTAA AACTGTCCTG CGCAATGAGC CTTTGGTTTT AGAGGGAGGC TATGAAAACT GGCTCCTTTG TTATCCCCAG TATACAACAA ATGCTAAGGT CACTCCACCC CCACGACGCC AGAATGAAGA GGTGTCTATC TCATTGGATT TTACTTATCC CTCATTGGAA GAATCAATTC CTTCTAAACC TGCTGCCCAG ACGCCACCTG CATCTATAGA AGTAGATGAA AATATAGAAT TGATAAGTGG TCAAAATGAG AGAATGGGAC CACTGAATAT ATCAACTCCA GTTGAACCAG TTGCTGCTTC TAAATCTGAT GTTTCACCCA TAATTCAGCC AGTGCCTAGT ATAAAGAATG TTCCACAGAT TGATCGTACT AAAAAACCAG CAGTCAAATT GCCTGAAGAG CATAGAATAA AATCTGAAAG TACAAACCAT GAGCAACAAT CTCCTCAGAG TGGAAAAGTT ATTCCTGATC GTTCCACCAA GCCAGTAGTT TTTTCTCCAA CTCTCATGTT AACAGATGAA GAAAAGGCTC GTATTCATGC AGAAACTGCT CTTCTAATGG AAAAAAACAA ACAAGAAAAA GAACTTCGGG AAAGGCAGCA AGAGGAACAG AAAGAGAAAC TGAGGAAGGA AGAACAAGAA CAAAAAGCCA AAAAGAAACA AGAAGCTGAA GAAAATGAAA TTACAGAGAA GCAACAAAAA GCAAAAGAAG AAATGGAGAA GAAAGAAAGT GAACAGGCCA AGAAAGAAGA TAAAGAAACC TCAGCAAAGA GGGGCAAAGA AATAACAGGA GTAAAAAGAC AAAGTAAAAG TGAACATGAA ACTTCTGATG CCAAGAAATC TGTAGAAGAT AGGGGGAAAA GGTGTCCAAC CCCAGAAATA CAGAAAAAGT CAACAGGAGA TGTGCCCCAT ACATCTGTGA CAGGGGATTC AGGTTCAGGC AAGCCATTTA AGATTAAAGG ACAACCAGAA AGTGGAATTC TAAGGACAGG AACTTTTAGA GAGGATACAG ACGATACCGA AAGAAATAAA GCTCAACGAG AACCTTTGAC AAGAGCACGA AGTGAAGAAA TGGGGAGGAT CGTACCAGGA CTGCCTTCAG GCTGGGCCAA GTTTCTTGAC CCAATCACTG GAACCTTTCG TTATTATCAT TCACCCACCA ACACTGTTCA TATGTACCCA CCGGAAATGG CTCCTTCATC TGCACCTCCT TCCACCCCTC CAACTCATAA AGCCAAGCCA CAGATTCCTG CTGAGCGGGA TAGGGAACCT TCCAAACTGA AGCGCTCCTA CTCCTCCCCA GATATAACCC AGGCTATTCA AGAGGAAGAG AAGAGGAAGC CAACAGTAAC TCCAACAGTT AATCGGGAAA ACAAGCCAAC ATGTTATCCT AAAGCTGAGA TCTCAAGGCT TTCTGCTTCT CAGATTCGGA ACCTCAATCC TGTTTTTGGA GGTTCTGGAC CAGCTCTTAC TGGACTTCGT AACTTAGGAA ATACTTGTTA TATGAACTCA ATATTGCAGT GCCTATGTAA CGCTCCACAT TTGGCTGATT ATTTCAACCG AAACTGTTAT CAGGATGATA TTAACAGGTC AAATTTGTTG GGGCATAAAG GTGAAGTGGC AGAAGAATTT GGTATAATCA TGAAAGCCCT GTGGACAGGA CAGTATAGAT ATATCAGTCC AAAGGACTTT AAAATCACCA TTGGGAAGAT CAATGACCAG TTTGCAGGAT ACAGTCAGCA AGATTCACAA GAATTGCTTC TGTTCCTAAT GGATGGTCTC CATGAAGATC TAAATAAAGC TGATAATCGG AAGAGATATA AAGAAGAAAA TAATGATCAT CTCGATGACT TTAAAGCTGC AGAACATGCC TGGCAGAAAC ACAAGCAGCT CAATGAGTCT ATTATTGTTG CACTTTTTCA GGGTCAATTC AAATCTACAG TACAGTGCCT CACATGTCAC AAAAAGTCTA GGACATTTGA GGCCTTCATG TATTTGTCTC TACCACTAGC ATCCACAAGT AAATGTACAT TACAGGATTG CCTTAGATTA TTTTCCAAAG AAGAAAAACT CACAGATAAC AACAGATTTT ACTGCAGTCA TTGCAGAGCT CGACGGGATT CTCTAAAAAA GATAGAAATC TGGAAGTTAC CACCTGTGCT TTTAGTGCAT CTGAAACGTT TTTCCTACGA TGGCAGGTGG AAACAAAAAT TACAGACATC TGTGGACTTC CCGTTAGAAA ATCTTGACTT GTCACAGTAT GTTATTGGTC CAAAGAACAA TTTGAAGAAA TATAATTTGT TTTCTGTTTC AAATCACTAC GGTGGGCTGG ATGGAGGCCA CTACACAGCC TATTGTAAAA ATGCAGCAAG ACAACGGTGG TTTAAGTTTG ATGATCATGA AGTTTCTGAT ATCTCCGTTT CTTCTGTGAA ATCTTCAGCA GCTTATATCC TCTTTTATAC TTCATTGGGA CCACGAGTAA CTGATGTAGC CACATAA. It is sometimes possible for the material contained within the vial of "USP8, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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