Universal Tissue cDNA, Reverse Transcribed by Random Hexamer, Canine Adult Normal, BioGenomics cDNA Clone
Universal Tissue cDNA, Reverse Transcribed by Random Hexamer, Canine Adult Normal, BioGenomics
Purity
Molecular Biology Grade
Synonyms
Universal Tissue cDNA; Reverse Transcribed by Random Hexamer; Canine Adult Normal; BioGenomics; BioGenomics cdna clone
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a liquid in 1X RT buffer: 50mM Tris-Cl, pH 8.3, 75mM KCl, 3mM MgCl2, 10mM DTT.
Application Notes
Suitable for use in SNP analysis, Southern Blot, PCR, Genomic DNA library construction, Profiling study in gene expression
Bioselect Tissue cDNA
cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.
BioSelect Universal cDNA Mixed Male and Female donors. Prepared by reverse transcribed RNA by using random hexamer or oligo dT primer. Total RNA is isolated by modified guanidine thiocyanate techniques. The Universal cDNA serves as a standard for comparison of gene expression by real time PCR and regular PCR, and also as a gene pool for cloning genes.
BioSelect Universal cDNA Mixed Male and Female donors. Prepared by reverse transcribed RNA by using random hexamer or oligo dT primer. Total RNA is isolated by modified guanidine thiocyanate techniques. The Universal cDNA serves as a standard for comparison of gene expression by real time PCR and regular PCR, and also as a gene pool for cloning genes.
Supplied With
T5595-0010: Tissue, cDNA, Actin Control Primer
Quality Control
RNA Integrity for cDNA synthesis examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA using denaturing agarose gel.
Quality and purity of total RNA is determined spectrophotometrically.
A260/280 1.8 - 2.0 (10mM Tris-Cl, pH 7.5)
8S/18S Ratio 1
• . RNA used for cDNA synthesis is treated by DNase I and is tested as DNA-free RNA by PCR.
• The synthesized human, animal, and cell line cDNA was 5' selected to ensure its full length.
• The cDNA was used as template for PCR amplification of beta-actin gene and an 838 bp beta-actin band was visualized on 1% agarose gel
• Synthesized plant cDNA was used as template for PCR amplification of chloroplast gene.
• 458bp chloroplast band visualized on 1% agarose gel.
-actin control primer included, 10 PCR reactions
Chloroplast control primer included., 10 PCR reactions
Quality and purity of total RNA is determined spectrophotometrically.
A260/280 1.8 - 2.0 (10mM Tris-Cl, pH 7.5)
8S/18S Ratio 1
• . RNA used for cDNA synthesis is treated by DNase I and is tested as DNA-free RNA by PCR.
• The synthesized human, animal, and cell line cDNA was 5' selected to ensure its full length.
• The cDNA was used as template for PCR amplification of beta-actin gene and an 838 bp beta-actin band was visualized on 1% agarose gel
• Synthesized plant cDNA was used as template for PCR amplification of chloroplast gene.
• 458bp chloroplast band visualized on 1% agarose gel.
-actin control primer included, 10 PCR reactions
Chloroplast control primer included., 10 PCR reactions
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for Universal Tissue cDNA, Reverse Transcribed by Random Hexamer, Canine Adult Normal, BioGenomics cdna clone
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Product Notes
The Universal Tissue cDNA, Reverse Transcribed by Random Hexamer, Canine Adult Normal, BioGenomics (Catalog #AAA652554) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in SNP analysis, Southern Blot, PCR, Genomic DNA library construction, Profiling study in gene expression. Researchers should empirically determine the suitability of the Universal Tissue cDNA, Reverse Transcribed by Random Hexamer, Canine Adult Normal, BioGenomics for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Universal Tissue cDNA, Reverse Transcribed by Random Hexamer, Canine Adult Normal, BioGenomics, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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