UHRF1 cdna clone
UHRF1 cDNA Clone
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014]
Uniprot Description
UHRF1: Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair. Defects in UHRF1 may be a cause of cancers. Overexpressed in many different forms of human cancers, including bladder, breast, cervical, colorectal and prostate cancers, as well as pancreatic adenocarcinomas, rhabdomyosarcomas and gliomas. Plays an important role in the correlation of histone modification and gene silencing in cancer progression. Expression is associated with a poor prognosis in patients with various cancers, suggesting that it participates in cancer progression. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; Ubiquitin ligase; EC 6.3.2.19; DNA-binding; EC 6.3.2.-; Transcription factor; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: euchromatin; heterochromatin; nuclear chromatin; nuclear heterochromatin; nuclear matrix; nucleus; replication fork
Molecular Function: histone binding; identical protein binding; methyl-CpG binding; methylated histone residue binding; nucleosomal histone binding; protein binding; transcription factor activity; ubiquitin-protein ligase activity; zinc ion binding
Biological Process: cell proliferation; histone monoubiquitination; histone ubiquitination; maintenance of DNA methylation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cellular protein metabolic process; positive regulation of transcription from RNA polymerase II promoter; protein autoubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process
Research Articles on UHRF1
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Product Notes
The UHRF1 uhrf1 (Catalog #AAA1271895) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: ATGGGGGTTT TTGCTGTCCC TCCCCTCAGC TCCGACACCA TGTGGATCCA GGTTCGGACC ATGGATGGGA GGCAGACCCA CACGGTGGAC TCGCTGTCCA GGCTGACCAA GGTGGAGGAG CTGAGGCGGA AGATCCAGGA GCTGTTCCAC GTGGAGCCAG GCCTGCAGAG GCTGTTCTAC AGGGGCAAAC AGATGGAGGA CGGCCATACC CTCTTCGACT ACGAGGTCCG CCTGAATGAC ACCATCCAGC TCCTGGTCCG CCAGAGCCTC GTGCTCCCCC ACAGCACCAA GGAGCGGGAC TCCGAGCTCT CCGACACCGA CTCCGGCTGC TGCCTGGGCC AGAGTGAGTC AGACAAGTCC TCCACCCACG GTGAGGCGGC CGCCGAGACT GACAGCAGGC CAGCCGATGA GGACATGTGG GATGAGACGG AATTGGGGCT GTACAAGGTC AATGAGTACG TCGATGCTCG GGACACGAAC ATGGGGGCGT GGTTTGAGGC GCAGGTGGTC AGGGTGACGC GGAAGGCCCC CTCCCGGGAC GAGCCCTGCA GCTCCACGTC CAGGCCGGCG CTGGAGGAGG ACGTCATTTA CCACGTGAAA TACGACGACT ACCCGGAGAA CGGCGTGGTC CAGATGAACT CCAGGGACGT CCGAGCGCGC GCCCGCACCA TCATCAAGTG GCAGGACCTG GAGGTGGGCC AGGTGGTCAT GCTCAACTAC AACCCCGACA ACCCCAAGGA GCGGGGCTTC TGGTACGACG CGGAGATCTC CAGGAAGCGC GAGACCAGGA CGGCGCGGGA ACTCTACGCC AACGTGGTGC TGGGGGATGA TTCTCTGAAC GACTGTCGGA TCATCTTCGT GGACGAAGTC TTCAAGATTG AGCGGCCGGG TGAAGGGAGC CCCATGGTTG ACAACCCCAT GAGACGGAAG AGCGGGCCGT CCTGCAAGCA CTGCAAGGAC GACGTGAACA GACTCTGCCG GGTCTGCGCC TGCCACCTGT GCGGGGGCCG GCAGGACCCC GACAAGCAGC TCATGTGCGA TGAGTGCGAC ATGGCCTTCC ACATCTACTG CCTGGACCCG CCCCTCAGCA GTGTTCCCAG CGAGGACGAG TGGTACTGCC CTGAGTGCCG GAATGATGCC AGCGAGGTGG TACTGGCGGG AGAGCGGCTG AGAGAGAGCA AGAAGAAGGC GAAGATGGCC TCGGCCACAT CGTCCTCACA GCGGGACTGG GGCAAGGGCA TGGCCTGTGT GGGCCGCACC AAGGAATGTA CCATCGTCCC GTCCAACCAC TACGGACCCA TCCCGGGGAT CCCCGTGGGC ACCATGTGGC GGTTCCGAGT CCAGGTCAGC GAGTCGGGTG TCCATCGGCC CCACGTGGCT GGCATACACG GCCGGAGCAA CGACGGAGCG TACTCCCTAG TCCTGGCGGG GGGCTATGAG GATGACGTGG ACCATGGGAA TTTTTTCACA TACACGGGTA GTGGTGGTCG AGATCTTTCC GGCAACAAGA GGACCGCGGA ACAGTCTTGT GATCAGAAAC TCACCAACAC CAACAGGGCG CTGGCTCTCA ACTGCTTTGC TCCCATCAAT GACCAAGAAG GGGCCGAGGC CAAGGACTGG CGGTCGGGGA AGCCGGTCAG GGTGGTGCGC AATGTCAAGG GTGGCAAGAA TAGCAAGTAC GCCCCCGCTG AGGGCAACCG CTATGATGGC ATCTACAAGG TTGTGAAATA CTGGCCCGAG AAGGGGAAGT CCGGGTTTCT CGTGTGGCGC TACCTTCTGC GGAGGGACGA TGATGAGCCT GGCCCTTGGA CGAAGGAGGG GAAGGACCGG ATCAAGAAGC TGGGGCTGAC CATGCAGTAT CCAGAAGGCT ACCTGGAAGC CCTGGCCAAC CGAGAGCGAG AGAAGGAGAA CAGCAAGAGG GAGGAGGAGG AGCAGCAGGA GGGGGGCTTC GCGTCCCCCA GGACGGGCAA GGGCAAGTGG AAGCGGAAGT CGGCAGGAGG TGGCCCGAGC AGGGCCGGGT CCCCGCGCCG GACATCCAAG AAAACCAAGG TGGAGCCCTA CAGTCTCACG GCCCAGCAGA GCAGCCTCAT CAGAGAGGAC AAGAGCAACG CCAAGCTGTG GAATGAGGTC CTGGCGTCAC TCAAGGACCG GCCGGCGAGC GGCAGCCCGT TCCAGTTGTT CCTGAGTAAA GTGGAGGAGA CGTTCCAGTG TATCTGCTGT CAGGAGCTGG TGTTCCGGCC CATCACGACC GTGTGCCAGC ACAACGTGTG CAAGGACTGC CTGGACAGAT CCTTTCGGGC ACAGGTGTTC AGCTGCCCTG CCTGCCGCTA CGACCTGGGC CGCAGCTATG CCATGCAGGT GAACCAGCCT CTGCAGACCG TCCTCAACCA GCTCTTCCCC GGCTACGGCA ATGGCCGGTG A. It is sometimes possible for the material contained within the vial of "UHRF1, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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