HAND1 cdna clone

HAND1

Cat. Num. AAA200532

• Gene Names: HAND1; Hxt; eHand; Thing1; bHLHa27
• Synonyms: HAND1; bHLHa27; eHand; Hxt; Thing1; HAND1 cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGAACCTCG TGGGCAGCTA CGCACACCAT CACCACCATC ACCACCCGCA CCCTGCGCAC CCCATGCTCC ACGAACCCTT CCTCTTCGGT CCGGCCTCGC GCTGTCATCA GGAAAGGCCC TACTTCCAGA GCTGGCTGCT GAGCCCGGCT GACGCTGCCC CGGACTTCCC TGCGGGCGGG CCGCCGCCCG CGGCCGCTGC AGCCGCCACC GCCTATGGTC CTGACGCCAG GCCTGGGCAG AGCCCCGGGC GGCTGGAGGC GCTTGGCGGC CGTCTTGGCC GGCGGAAAGG CTCAGGACCC AAGAAGGAGC GGAGACGCAC TGAGAGCATT AACAGCGCAT TCGCGGAGTT GCGCGAGTGC ATCCCCAACG TGCCGGCCGA CACCAAGCTC TCCAAGATCA AGACTCTGCG CCTAGCCACC AGCTACATCG CCTACCTGAT GGACGTGCTG GCCAAGGATG CACAGTCTGG CGATCCCGAG GCCTTCAAGG CTGAACTCAA GAAGGCGGAT GGCGGCCGTG AGAGCAAGCG GAAAAGGGAG CTGCAGCAGC ACGAAGGTTT TCCTCCTGCC CTGGGCCCAG TCGAGAAGAG GATTAAAGGA CGCACCGGCT GGCCGCAGCA AGTCTGGGCG CTGGAGTTAA ACCAGTGA; ; Translation Sequence: MNLVGSYAHH HHHHHPHPAH PMLHEPFLFG PASRCHQERP YFQSWLLSPA DAAPDFPAGG PPPAAAAAAT AYGPDARPGQ SPGRLEALGG RLGRRKGSGP KKERRRTESI NSAFAELREC IPNVPADTKL SKIKTLRLAT SYIAYLMDVL AKDAQSGDPE AFKAELKKAD GGRESKRKRE LQQHEGFPPA LGPVEKRIKG RTGWPQQVWA LELNQ
• Sequence Length: 215

• Species: Human
• Chromosome Location: 5q33
• OMIM Reference Number: 602406
• cDNA Size: 648bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for HAND1 cdna clone

HAND1 belongs to the basic helix-loop-helix family of transcription factors. This protein is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.

Product Categories/Family for HAND1 cdna clone

Development

NCBI and Uniprot Product Information

• NCBI GI #: 4758506
• NCBI GeneID: 9421
• NCBI Accession #: NP_004812.1
• NCBI GenBank Nucleotide #: NM_004821.2
• UniProt Accession #: O96004
• NCBI Official Full Name: heart- and neural crest derivatives-expressed protein 1
• NCBI Official Synonym Full Names: heart and neural crest derivatives expressed 1
• NCBI Official Symbol: HAND1
• NCBI Official Synonym Symbols: Hxt; eHand; Thing1; bHLHa27
• NCBI Protein Information: heart- and neural crest derivatives-expressed protein 1
• UniProt Protein Name: Heart- and neural crest derivatives-expressed protein 1
• Protein Family: Heart- and neural crest derivatives-expressed protein
• UniProt Gene Name: HAND1
• UniProt Synonym Gene Names: BHLHA27; EHAND; bHLHa27; eHAND
• UniProt Entry Name: HAND1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

HAND1: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'- NRTCTG-3' (non-canonical E-box).

Protein type: Transcription factor; Nucleolus; Cell development/differentiation; DNA-binding

Chromosomal Location of Human Ortholog: 5q33

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; enzyme binding; protein heterodimerization activity; transcription coactivator activity; bHLH transcription factor binding; transcription factor binding; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; blastocyst development; negative regulation of transcription factor activity; heart development; negative regulation of transcription from RNA polymerase II promoter; odontogenesis of dentine-containing teeth; trophectodermal cell differentiation; mesoderm formation; ventricular cardiac muscle morphogenesis; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; heart looping; negative regulation of transcription, DNA-dependent