EIF4A3 cdna clone
EIF4A3 cDNA Clone
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq, Jul 2008]
Uniprot Description
DDX48: ATP-dependent RNA helicase. Component of a splicing- dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Belongs to the DEAD box helicase family. eIF4A subfamily.
Protein type: Spliceosome; RNA-binding; EC 3.6.4.13; Helicase; RNA splicing
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm
Molecular Function: ATP binding; ATP-dependent RNA helicase activity; mRNA binding; poly(A) binding; protein binding
Biological Process: embryonic cranial skeleton morphogenesis; mRNA 3'-end processing; mRNA catabolic process, nonsense-mediated decay; mRNA export from nucleus; negative regulation of translation; nuclear mRNA splicing, via spliceosome; poly(A) tail shortening; positive regulation of translation; RNA export from nucleus; RNA secondary structure unwinding; RNA splicing; termination of RNA polymerase II transcription
Disease: Robin Sequence With Cleft Mandible And Limb Anomalies
Research Articles on EIF4A3
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Product Notes
The EIF4A3 eif4a3 (Catalog #AAA1276385) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: atggcgacca cggccacgat ggcgacctcg ggctcggcgc gaaagcggct gctcaaagag gaagacatga ctaaagtgga attcgagacc agcgaggagg tggatgtgac ccccacgttc gacaccatgg gcctgcggga ggacctgctg cggggcatct acgcttacgg ttttgaaaaa ccatcagcaa tccagcaacg agcaatcaag cagatcatca aagggagaga tgtcatcgca cagtctcagt ccggcacagg aaaaacagcc accttcagta tctcagtcct ccagtgtttg gatattcagg ttcgtgaaac tcaagctttg atcttggctc ccacaagaga gttggctgtg cagatccaga aggggctgct tgctctcggt gactacatga atgtccagtg ccatgcctgc attggaggca ccaatgttgg cgaggacatc aggaagctgg attacggaca gcatgttgtc gcgggcactc cagggcgtgt ttttgatatg attcgtcgca gaagcctaag gacacgtgct atcaaaatgt tggttttgga tgaagctgat gaaatgttga ataaaggttt caaagagcag atttacgatg tatacaggta cctgcctcca gccacacagg tggttctcat cagtgccacg ctgccacacg agattctgga gatgaccaac aagttcatga ccgacccaat ccgcatcttg gtgaaacgtg atgaattgac tctggaaggc atcaagcaat ttttcgtggc agtggagagg gaagagtgga aatttgacac tctgtgtgac ctctacgaca cactgaccat cactcaggcg gtcatcttct gcaacaccaa aagaaaggtg gactggctga cggagaaaat gagggaagcc aacttcactg tatcctcaat gcatggagac atgccccaga aagagcggga gtccatcatg aaggagttcc ggtcgggcgc cagccgagtg cttatttcta cagatgtctg ggccaggggg ttggatgtcc ctcaggtgtc cctcatcatt aactatgatc tccctaataa cagagaattg tacatacaca gaattgggag atcaggtcga tacggccgga agggtgtggc cattaacttt gtaaagaatg acgacatccg catcctcaga gatatcgagc agtactattc cactcagatt gatgagatgc cgatgaacgt tgctgatctt atctga. It is sometimes possible for the material contained within the vial of "EIF4A3, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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